On-chip formation of 3D spheroids for patient-derived tissue screening

T. Christ, S. Payne, Y. Yan, J. Ren, J. Edwards, M. Boyd, M. Zagnoni

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.
LanguageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of PublicationSan Diego, CA
Pages577-578
Number of pages2
Publication statusPublished - 9 Oct 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated titleMicroTas 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Fingerprint

Biopsy
Microchannels
Microfluidics
Tumors
Screening
Personnel
Tissue
Monitoring

Keywords

  • spheroid tumour
  • drug-screening
  • cancer research
  • microfluidic device
  • 3D multicellular spheroids
  • biopsy samples

Cite this

Christ, T., Payne, S., Yan, Y., Ren, J., Edwards, J., Boyd, M., & Zagnoni, M. (2016). On-chip formation of 3D spheroids for patient-derived tissue screening. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 577-578). San Diego, CA.
Christ, T. ; Payne, S. ; Yan, Y. ; Ren, J. ; Edwards, J. ; Boyd, M. ; Zagnoni, M. / On-chip formation of 3D spheroids for patient-derived tissue screening. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. pp. 577-578
@inproceedings{5ab619a0a50a4eee8fb00d559a9e096e,
title = "On-chip formation of 3D spheroids for patient-derived tissue screening",
abstract = "Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.",
keywords = "spheroid tumour, drug-screening, cancer research, microfluidic device, 3D multicellular spheroids, biopsy samples",
author = "T. Christ and S. Payne and Y. Yan and J. Ren and J. Edwards and M. Boyd and M. Zagnoni",
year = "2016",
month = "10",
day = "9",
language = "English",
isbn = "9781510834163",
pages = "577--578",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

}

Christ, T, Payne, S, Yan, Y, Ren, J, Edwards, J, Boyd, M & Zagnoni, M 2016, On-chip formation of 3D spheroids for patient-derived tissue screening. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, pp. 577-578, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Dublin, Ireland, 9/10/16.

On-chip formation of 3D spheroids for patient-derived tissue screening. / Christ, T.; Payne, S.; Yan, Y.; Ren, J.; Edwards, J.; Boyd, M.; Zagnoni, M.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. p. 577-578.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

T1 - On-chip formation of 3D spheroids for patient-derived tissue screening

AU - Christ, T.

AU - Payne, S.

AU - Yan, Y.

AU - Ren, J.

AU - Edwards, J.

AU - Boyd, M.

AU - Zagnoni, M.

PY - 2016/10/9

Y1 - 2016/10/9

N2 - Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.

AB - Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.

KW - spheroid tumour

KW - drug-screening

KW - cancer research

KW - microfluidic device

KW - 3D multicellular spheroids

KW - biopsy samples

UR - https://cbmsociety.org/conferences/

UR - http://microtas2016.org/

M3 - Conference contribution book

SN - 9781510834163

SP - 577

EP - 578

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

CY - San Diego, CA

ER -

Christ T, Payne S, Yan Y, Ren J, Edwards J, Boyd M et al. On-chip formation of 3D spheroids for patient-derived tissue screening. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA. 2016. p. 577-578