Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research . However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.
|Title of host publication||20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)|
|Place of Publication||San Diego, CA|
|Number of pages||2|
|Publication status||Published - 9 Oct 2016|
|Event||20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland|
Duration: 9 Oct 2016 → 13 Oct 2016
|Conference||20th International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Abbreviated title||MicroTas 2016|
|Period||9/10/16 → 13/10/16|
- spheroid tumour
- cancer research
- microfluidic device
- 3D multicellular spheroids
- biopsy samples
Christ, T., Payne, S., Yan, Y., Ren, J., Edwards, J., Boyd, M., & Zagnoni, M. (2016). On-chip formation of 3D spheroids for patient-derived tissue screening. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 577-578). San Diego, CA.