On-chip formation of 3D spheroids for patient-derived tissue screening

T. Christ; S. Payne; Y. Yan; J. Ren; J. Edwards; M. Boyd; M. Zagnoni

On-chip formation of 3D spheroids for patient-derived tissue screening

T. Christ, S. Payne, Y. Yan, J. Ren, J. Edwards, M. Boyd, M. Zagnoni

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

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Abstract

Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of Publication	San Diego, CA
Pages	577-578
Number of pages	2
Publication status	Published - 9 Oct 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 http://www.microtas2016.org/

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTas 2016
Country	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://www.microtas2016.org/

Keywords

spheroid tumour
drug-screening
cancer research
microfluidic device
3D multicellular spheroids
biopsy samples

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Cite this

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title = "On-chip formation of 3D spheroids for patient-derived tissue screening",

abstract = "Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network. ",

keywords = "spheroid tumour, drug-screening, cancer research, microfluidic device, 3D multicellular spheroids, biopsy samples",

author = "T. Christ and S. Payne and Y. Yan and J. Ren and J. Edwards and M. Boyd and M. Zagnoni",

year = "2016",

month = oct,

day = "9",

language = "English",

isbn = "9781510834163",

pages = "577--578",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

url = "http://www.microtas2016.org/",

}

Christ, T, Payne, S, Yan, Y, Ren, J, Edwards, J, Boyd, M & Zagnoni, M 2016, On-chip formation of 3D spheroids for patient-derived tissue screening. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, pp. 577-578, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Dublin, Ireland, 9/10/16.

On-chip formation of 3D spheroids for patient-derived tissue screening. / Christ, T.; Payne, S.; Yan, Y.; Ren, J.; Edwards, J.; Boyd, M.; Zagnoni, M.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. p. 577-578.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

TY - GEN

T1 - On-chip formation of 3D spheroids for patient-derived tissue screening

AU - Christ, T.

AU - Payne, S.

AU - Yan, Y.

AU - Ren, J.

AU - Edwards, J.

AU - Boyd, M.

AU - Zagnoni, M.

PY - 2016/10/9

Y1 - 2016/10/9

N2 - Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.

AB - Three-dimensional (3D) multicellular spheroids have been identified as a suitable model of solid tumours for drug screening and cancer research [1]. However, creating and monitoring a large number of spheroids using conventional methods remains labour-intensive. This work reports a novel double-layer microfluidic device for the generation and culture of 3D multicellular spheroids and its use with biopsy samples. The system enables drug concentration-response curves to be obtained from a limited amount of sample material by applying a drug concentration gradient using an integrated passive microchannel network.

KW - spheroid tumour

KW - drug-screening

KW - cancer research

KW - microfluidic device

KW - 3D multicellular spheroids

KW - biopsy samples

UR - https://cbmsociety.org/conferences/

UR - http://microtas2016.org/

M3 - Conference contribution book

SN - 9781510834163

SP - 577

EP - 578

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

CY - San Diego, CA

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 9 October 2016 through 13 October 2016

ER -

On-chip formation of 3D spheroids for patient-derived tissue screening

Abstract

Conference

Keywords

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