Oligonucleotide conjugation to a cell-penetrating (tat) peptide by diels-alder cycloaddition

V. Steven, D. Graham

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Modifed oligonucleotides are routinely employed as analytical probes for use in diagnostics, e.g. in the examination of specific RNA sequences for infectious diseases, however, a major limiting factor in oligonucleotide-based diagnostics is poor cellular uptake of naked oligonucleotides. This problem can be overcome by covalent attachment of a so-called 'cell-penetrating peptide' to form an oligonucleotide peptide conjugate. Stepwise solid phase synthesis of such a conjugate is difficult and expensive due to the conflicting chemistries of oligonucleotides and peptides. A simple approach to overcome this is post-synthetic conjugation. Diels-Alder cycloaddition is an attractive methodology for oligonucleotide peptide conjugation; the reaction is fast, chemoselective and the reaction rate is greatly enhanced in aqueous media - ideal conditions for biological moieties. An oligodeoxyribonucleotide sequence has been derivatised with a series of dienes at the 5′-terminus, using a series of unique dienyl-modified phosphoramidites, and investigation into the effect of diene type on the efficiency of conjugation, using Diels-Alder cycloaddition with a maleimido-derivatised cell-penetrating (TAT) peptide, has been performed. This led to the observation that the optimal diene for conjugation was cyclohexadiene, allowing conjugation of oligodeoxyribonucleotides to a cell-penetrating peptide by Diels-Alder cycloaddition for the first time. (Taken from Swetswise web site: http://www.swetswise.com/eAccess/viewAbstract.do?articleID=38485673)
Original languageEnglish
Pages (from-to)3781-3787
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume6
Issue number20
DOIs
Publication statusPublished - 21 Oct 2008

Keywords

  • oligonucleotide
  • conjugation
  • cell-penetrating (tat) peptide
  • diels-alder cycloaddition

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