Projects per year
Abstract
Stimuli-responsive nanocarriers have become increasingly important for nucleic acid and drug delivery in cancer therapy. Here, we report the synthesis, characterization and evaluation of disulphide-linked, octadecyl (C18 alkyl) chain-bearing PEGylated generation 3-diaminobutyric polypropylenimine dendrimer-based vesicles (or dendrimersomes) for gene delivery. The lipid-bearing PEGylated dendrimer was successfully synthesized through in situ two-step reaction. It was able to spontaneously self-Assemble into stable, cationic, nanosized vesicles, with low critical aggregation concentration value, and also showed redox-responsiveness in presence of a glutathione concentration similar to that of the cytosolic reducing environment. In addition, it was able to condense more than 70% of DNA at dendrimer: DNA weight ratios of 5 : 1 and higher. This dendriplex resulted in an enhanced cellular uptake of DNA at dendrimer: DNA weight ratios of 10 : 1 and 20 : 1, by up to 16-fold and by up to 28-fold compared with naked DNA in PC-3 and DU145 prostate cancer cell lines respectively. At a dendrimer: DNA weight ratio of 20 : 1, it led to an increase in gene expression in PC-3 and DU145 cells, compared with DAB dendriplex. These octadecyl chain-bearing, PEGylated dendrimer-based vesicles are therefore promising redox-sensitive drug and gene delivery systems for potential applications in combination cancer therapy. This journal is
Original language | English |
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Pages (from-to) | 1431-1448 |
Number of pages | 18 |
Journal | Biomaterials Science |
Volume | 9 |
Issue number | 4 |
Early online date | 22 Dec 2020 |
DOIs | |
Publication status | Published - 21 Feb 2021 |
Keywords
- octadecyl (C18 alkyl) chain
- cancer
- gene delivery
- redox sensitivity
- dendrimersomes
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Dive into the research topics of 'Octadecyl chain-bearing PEGylated poly(propyleneimine)-based dendrimersomes: physicochemical studies, redox-responsiveness, DNA condensation, cytotoxicity and gene delivery to cancer cells'. Together they form a unique fingerprint.Projects
- 2 Finished
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Development of targeted nanomedicines to treat prostate cancer
Worldwide Cancer Research (formerly AICR)
1/06/16 → 30/11/19
Project: Research
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Development of novel gene delivery systems for brain targeting by intravenous administration
1/05/16 → 30/04/19
Project: Research