Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS)

Natalie Clark, K. Faulds, D. Graham

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.
LanguageEnglish
Title of host publicationDNA conjugates and sensors
EditorsStephen Neidle, Keith R Fox , Tom Brown
Pages258-277
Number of pages20
DOIs
Publication statusPublished - 1 Jan 2012

Publication series

NameRSC Biomolecular Sciences
PublisherRSC Publishing

Fingerprint

Nucleic Acids
Raman scattering
Nanoparticles
Sensors
DNA
Fluorescence
Molecules
Labels
Monitoring
Substrates

Keywords

  • surface enhanced resonance raman scattering
  • nucleic acid-nanoparticle
  • conjugate sensors
  • SERRS

Cite this

Clark, N., Faulds, K., & Graham, D. (2012). Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). In S. Neidle, K. R. Fox , & T. Brown (Eds.), DNA conjugates and sensors (pp. 258-277). (RSC Biomolecular Sciences). https://doi.org/10.1039/9781849734936-00258
Clark, Natalie ; Faulds, K. ; Graham, D. / Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). DNA conjugates and sensors. editor / Stephen Neidle ; Keith R Fox ; Tom Brown. 2012. pp. 258-277 (RSC Biomolecular Sciences).
@inbook{efef05a6737447b997aaa2d903c8ee74,
title = "Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS)",
abstract = "The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.",
keywords = "surface enhanced resonance raman scattering , nucleic acid-nanoparticle, conjugate sensors, SERRS",
author = "Natalie Clark and K. Faulds and D. Graham",
year = "2012",
month = "1",
day = "1",
doi = "10.1039/9781849734936-00258",
language = "English",
isbn = "9781849734271",
series = "RSC Biomolecular Sciences",
publisher = "RSC Publishing",
pages = "258--277",
editor = "Neidle, {Stephen } and {Fox }, {Keith R } and Brown, {Tom }",
booktitle = "DNA conjugates and sensors",

}

Clark, N, Faulds, K & Graham, D 2012, Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). in S Neidle, KR Fox & T Brown (eds), DNA conjugates and sensors. RSC Biomolecular Sciences, pp. 258-277. https://doi.org/10.1039/9781849734936-00258

Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). / Clark, Natalie; Faulds, K.; Graham, D.

DNA conjugates and sensors. ed. / Stephen Neidle; Keith R Fox ; Tom Brown. 2012. p. 258-277 (RSC Biomolecular Sciences).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS)

AU - Clark, Natalie

AU - Faulds, K.

AU - Graham, D.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.

AB - The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.

KW - surface enhanced resonance raman scattering

KW - nucleic acid-nanoparticle

KW - conjugate sensors

KW - SERRS

UR - http://www.scopus.com/inward/record.url?scp=84872454118&partnerID=8YFLogxK

UR - http://pubs.rsc.org/en/content/chapter/bk9781849734271-00258/978-1-84973-427-1#!divabstract

U2 - 10.1039/9781849734936-00258

DO - 10.1039/9781849734936-00258

M3 - Chapter

SN - 9781849734271

T3 - RSC Biomolecular Sciences

SP - 258

EP - 277

BT - DNA conjugates and sensors

A2 - Neidle, Stephen

A2 - Fox , Keith R

A2 - Brown, Tom

ER -

Clark N, Faulds K, Graham D. Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). In Neidle S, Fox KR, Brown T, editors, DNA conjugates and sensors. 2012. p. 258-277. (RSC Biomolecular Sciences). https://doi.org/10.1039/9781849734936-00258