Abstract
| Language | English |
|---|---|
| Title of host publication | DNA conjugates and sensors |
| Editors | Stephen Neidle, Keith R Fox , Tom Brown |
| Pages | 258-277 |
| Number of pages | 20 |
| DOIs | |
| Publication status | Published - 1 Jan 2012 |
Publication series
| Name | RSC Biomolecular Sciences |
|---|---|
| Publisher | RSC Publishing |
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Keywords
- surface enhanced resonance raman scattering
- nucleic acid-nanoparticle
- conjugate sensors
- SERRS
Cite this
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Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS). / Clark, Natalie; Faulds, K.; Graham, D.
DNA conjugates and sensors. ed. / Stephen Neidle; Keith R Fox ; Tom Brown. 2012. p. 258-277 (RSC Biomolecular Sciences).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Nucleic acid-nanoparticle conjugate sensors for use with surface enhanced resonance raman scattering (SERRS)
AU - Clark, Natalie
AU - Faulds, K.
AU - Graham, D.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.
AB - The ability to detect DNA relating to a specific disease is at the forefront of current research in molecular diagnostics. The detection techniques utilised for such work need to afford excellent selectivity as well as sensitivity. Current methods commonly involve the use of fluorescence, however there has been a heightened interest in the use of surface-enhanced resonance Raman scattering [SE(R)RS] for these purposes in recent years because of the numerous advantages SE(R)RS offer over fluorescence. Nanoparticles are often selected as the SE(R)RS substrate of choice. These nanoparticles can undergo a number of functionalisation steps in order for them either to act as labels for detection, to detect specific target molecules, or to perform both tasks simultaneously. These SE(R)RS detection techniques can be performed in two ways: by the use of small molecules to produce nanoparticle-tags, or by monitoring a nanoparticle assembly process that results in a signal. This chapter focuses on the use of DNA–nanoparticle conjugates and their use for DNA detection by SE(R)RS.
KW - surface enhanced resonance raman scattering
KW - nucleic acid-nanoparticle
KW - conjugate sensors
KW - SERRS
UR - http://www.scopus.com/inward/record.url?scp=84872454118&partnerID=8YFLogxK
UR - http://pubs.rsc.org/en/content/chapter/bk9781849734271-00258/978-1-84973-427-1#!divabstract
U2 - 10.1039/9781849734936-00258
DO - 10.1039/9781849734936-00258
M3 - Chapter
SN - 9781849734271
T3 - RSC Biomolecular Sciences
SP - 258
EP - 277
BT - DNA conjugates and sensors
A2 - Neidle, Stephen
A2 - Fox , Keith R
A2 - Brown, Tom
ER -