Nucleic acid-induced aggregation of metal nanoparticles and uses thereof in methods for detecting nucleic acids

Ramon Alvarez-Puebla (Inventor), Luca Guerrini (Inventor), Juan Sagalés Mañas (Inventor), Duncan Graham (Inventor)

Research output: Patent

Abstract

The invention relates to an aggregate comprising metallic nanoparticles and nucleic acid molecules wherein each metallic nanoparticle is coated with a polycation. The invention also relates to the a method for obtaining the aggregate of the invention and to the use of said aggregate in methods for detecting the presence of a nucleic acid in a sample, in methods for detecting the presence of a given nucleotide at a predetermined position in a target nucleic acid, in methods for detecting the presence of a modified nucleotide at a predetermined position in a target nucleic acid, methods for detecting the presence of a conjugate between a double stranded nucleic acid and a chemical in a sample comprising double stranded nucleic acid molecules, in methods for determining the content of modified nucleotides in a target nucleic acid and in method for determining the content of modified nucleotides in a target nucleic acid.
LanguageEnglish
Priority date23/10/14
Publication statusPublished - 27 Apr 2016

Fingerprint

Metal nanoparticles
Nucleic Acids
Agglomeration
Patents and inventions
Nucleotides
Nanoparticles
Molecules

Keywords

  • spectroscopy
  • nucleic acid
  • SERS
  • metallic nanoparticle

Cite this

Alvarez-Puebla, Ramon (Inventor) ; Guerrini, Luca (Inventor) ; Sagalés Mañas, Juan (Inventor) ; Graham, Duncan (Inventor). / Nucleic acid-induced aggregation of metal nanoparticles and uses thereof in methods for detecting nucleic acids.
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title = "Nucleic acid-induced aggregation of metal nanoparticles and uses thereof in methods for detecting nucleic acids",
abstract = "The invention relates to an aggregate comprising metallic nanoparticles and nucleic acid molecules wherein each metallic nanoparticle is coated with a polycation. The invention also relates to the a method for obtaining the aggregate of the invention and to the use of said aggregate in methods for detecting the presence of a nucleic acid in a sample, in methods for detecting the presence of a given nucleotide at a predetermined position in a target nucleic acid, in methods for detecting the presence of a modified nucleotide at a predetermined position in a target nucleic acid, methods for detecting the presence of a conjugate between a double stranded nucleic acid and a chemical in a sample comprising double stranded nucleic acid molecules, in methods for determining the content of modified nucleotides in a target nucleic acid and in method for determining the content of modified nucleotides in a target nucleic acid.",
keywords = "spectroscopy , nucleic acid, SERS, metallic nanoparticle",
author = "Ramon Alvarez-Puebla and Luca Guerrini and {Sagal{\'e}s Ma{\~n}as}, Juan and Duncan Graham",
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Nucleic acid-induced aggregation of metal nanoparticles and uses thereof in methods for detecting nucleic acids. / Alvarez-Puebla, Ramon (Inventor); Guerrini, Luca (Inventor); Sagalés Mañas, Juan (Inventor); Graham, Duncan (Inventor).

Research output: Patent

TY - PAT

T1 - Nucleic acid-induced aggregation of metal nanoparticles and uses thereof in methods for detecting nucleic acids

AU - Alvarez-Puebla, Ramon

AU - Guerrini, Luca

AU - Sagalés Mañas, Juan

AU - Graham, Duncan

PY - 2016/4/27

Y1 - 2016/4/27

N2 - The invention relates to an aggregate comprising metallic nanoparticles and nucleic acid molecules wherein each metallic nanoparticle is coated with a polycation. The invention also relates to the a method for obtaining the aggregate of the invention and to the use of said aggregate in methods for detecting the presence of a nucleic acid in a sample, in methods for detecting the presence of a given nucleotide at a predetermined position in a target nucleic acid, in methods for detecting the presence of a modified nucleotide at a predetermined position in a target nucleic acid, methods for detecting the presence of a conjugate between a double stranded nucleic acid and a chemical in a sample comprising double stranded nucleic acid molecules, in methods for determining the content of modified nucleotides in a target nucleic acid and in method for determining the content of modified nucleotides in a target nucleic acid.

AB - The invention relates to an aggregate comprising metallic nanoparticles and nucleic acid molecules wherein each metallic nanoparticle is coated with a polycation. The invention also relates to the a method for obtaining the aggregate of the invention and to the use of said aggregate in methods for detecting the presence of a nucleic acid in a sample, in methods for detecting the presence of a given nucleotide at a predetermined position in a target nucleic acid, in methods for detecting the presence of a modified nucleotide at a predetermined position in a target nucleic acid, methods for detecting the presence of a conjugate between a double stranded nucleic acid and a chemical in a sample comprising double stranded nucleic acid molecules, in methods for determining the content of modified nucleotides in a target nucleic acid and in method for determining the content of modified nucleotides in a target nucleic acid.

KW - spectroscopy

KW - nucleic acid

KW - SERS

KW - metallic nanoparticle

UR - https://patents.google.com/patent/EP3012221A1/en

M3 - Patent

ER -