Abstract
The invention relates to an aggregate comprising metallic nanoparticles and nucleic acid molecules wherein each metallic nanoparticle is coated with a polycation. The invention also relates to the a method for obtaining the aggregate of the invention and to the use of said aggregate in methods for detecting the presence of a nucleic acid in a sample, in methods for detecting the presence of a given nucleotide at a predetermined position in a target nucleic acid, in methods for detecting the presence of a modified nucleotide at a predetermined position in a target nucleic acid, methods for detecting the presence of a conjugate between a double stranded nucleic acid and a chemical in a sample comprising double stranded nucleic acid molecules, in methods for determining the content of modified nucleotides in a target nucleic acid and in method for determining the content of modified nucleotides in a target nucleic acid.
| Original language | English |
|---|---|
| Priority date | 23/10/14 |
| Publication status | Published - 27 Apr 2016 |
Keywords
- spectroscopy
- nucleic acid
- SERS
- metallic nanoparticle