Novel screening method for enzyme activity and enantioselectivity using surface enhanced resonance Raman scattering

L.C. Stevenson, D. Graham, B.D. Moore

Research output: Contribution to journalArticle

Abstract

The use of surface enhanced resonance Raman scattering (SERRS) as a screening method for enzyme catalysed reactions is reported. Sensitive and reproducible detection has been possible, giving information on both enzyme activity and the enantioselectivity of the enzyme. Surface enhanced resonance Raman scattering (SERRS) [1-3] is one of the most sensitive spectroscopic techniques for molecular detection. To exploit this sensitivity, benzotriazole based dyes have been developed as optimal labels for detection and analysis using SERRS [4] as they adsorb strongly and irreversibly onto metal surfaces [5]. Masked benzotriazole dyes (see Figure 1) were designed to be completely SERRS inactive, thus facilitating development of benzotriazole derivatives which can be cleaved via an enzyme-catalysed reaction to release the SERRS active benzotriazole group.
Original languageEnglish
Pages (from-to)U243-U244
JournalAbstracts of papers - American Chemical Society
Volume225
Issue number368-BIOT Part 1
Publication statusPublished - Mar 2003

Keywords

  • surface enhanced resonance raman scattering
  • enzyme activity
  • enantioselectivity

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