Novel polymer platform for enhanced biochip performance

H. M. McEvoy, R. Blue, N. Kent, Lubos Polerecky, C. McDonagh, B. D. MacCraith

Research output: Contribution to journalConference Contribution

1 Citation (Scopus)

Abstract

We report the development of enhanced optical platforms for fluorescence-based biosensors. A previous analysis by us has shown that the emission of fluorescence in such a system is highly anisotropic and is preferentially emitted into the substrate over a well-defined angular range, with the result that the light is guided along the substrate via total internal reflection. However, conventional optical biosensors based on fluorescence detection typically employ a detector that is positioned either directly above or directly below the biochip. As a consequence, only a small fraction of the total emitted fluorescence is detected, which impacts adversely on sensor performance. The enhanced biosensor presented here is based on a novel, generic platform specifically designed to overcome the inherent limitations of planar substrates. The platform incorporates custom-designed optical elements, the purpose of which is to redirect the emitted fluorescence onto a detector positioned beneath the biochip. Platforms were fabricated using the polymer processing technique of microinjection moulding. In this paper we demonstrate the ability of this optical system to achieve a 80-fold luminescence capture enhancement. We also demonstrate its effectiveness as an enhanced biosensor platform by carrying out a proof of principle BSA/antiBSA competitive assay. This work has significant implications for the development of mass-producible, highly efficient optical biosensors.
Original languageEnglish
Pages (from-to)512-519
Number of pages8
JournalProceedings of SPIE
Volume5826
DOIs
Publication statusPublished - 14 Jul 2005
EventProceedings of SPIE (volume 5826): Opto-Ireland 2005: Optical Sensing and Spectroscopy - Dublin, Ireland
Duration: 4 Apr 2005 → …

Keywords

  • novel polymer platform
  • enhanced biochip performance
  • sensor performance
  • sensors
  • biosensors
  • fluorescence spectroscopy
  • luminescence
  • optical biosensors
  • optical components
  • optical systems
  • polymers
  • reflection

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  • Cite this

    McEvoy, H. M., Blue, R., Kent, N., Polerecky, L., McDonagh, C., & MacCraith, B. D. (2005). Novel polymer platform for enhanced biochip performance. Proceedings of SPIE, 5826, 512-519. https://doi.org/10.1117/12.606423