Novel nanocomposites from spider silk-silica fusion (chimeric) proteins

C W P Foo, S V Patwardhan, D J Belton, B Kitchel, D Anastasiades, J Huang, R R Naik, C C Perry, D L Kaplan

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Silica skeletal architectures in diatoms are characterized by remarkable morphological and nanostructural details. Silk proteins from spiders and silkworms form strong and intricate self-assembling fibrous biomaterials in nature. We combined the features of silk with biosilica through the design, synthesis, and characterization of a novel family of chimeric proteins for subsequent use in model materials forming reactions. The domains from the major ampullate spidroin 1 (MaSp1) protein of Nephila clavipes spider dragline silk provide control over structural and morphological details because it can be self-assembled through diverse processing methods including film casting and fiber electrospinning. Biosilica nanostructures in diatoms are formed in aqueous ambient conditions at neutral pH and low temperatures. The R5 peptide derived from the silaffin protein of Cylindrotheca fusiformis induces and regulates silica precipitation in the chimeric protein designs under similar ambient conditions. Whereas mineralization reactions performed in the presence of R5 peptide alone form silica particles with a size distribution of 0.5-10 mu m in diameter, reactions performed in the presence of the new fusion proteins generate nanocomposite materials containing silica particles with a narrower size distribution of 0.5-2 mu m in diameter. Furthermore, we demonstrate that composite morphology and structure could be regulated by controlling processing conditions to produce films and fibers. These results suggest that the chimeric protein provides new options for processing and control over silica particle sizes, important benefits for biomedical and specialty materials, particularly in light of the all aqueous processing and the nanocomposite features of these new materials.

LanguageEnglish
Pages9428-9433
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume103
Issue number25
DOIs
Publication statusPublished - 20 Jun 2006

Fingerprint

Silk
Silicon Dioxide
Nanocomposites
Fusion reactions
Proteins
Processing
Arthropod Proteins
Peptides
Fibers
Electrospinning
Biocompatible Materials
Nanostructures
Casting
Particle size
Composite materials

Keywords

  • biomaterials
  • nanostructures
  • silaffin
  • biomineralization
  • ceramics
  • BIOACTIVE COMPOSITE-MATERIALS
  • fibroin
  • silicification
  • morphogenesis
  • crystals

Cite this

Foo, C. W. P., Patwardhan, S. V., Belton, D. J., Kitchel, B., Anastasiades, D., Huang, J., ... Kaplan, D. L. (2006). Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. Proceedings of the National Academy of Sciences , 103(25), 9428-9433. https://doi.org/10.1073/pnas.0601096103
Foo, C W P ; Patwardhan, S V ; Belton, D J ; Kitchel, B ; Anastasiades, D ; Huang, J ; Naik, R R ; Perry, C C ; Kaplan, D L . / Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. In: Proceedings of the National Academy of Sciences . 2006 ; Vol. 103, No. 25. pp. 9428-9433.
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Foo, CWP, Patwardhan, SV, Belton, DJ, Kitchel, B, Anastasiades, D, Huang, J, Naik, RR, Perry, CC & Kaplan, DL 2006, 'Novel nanocomposites from spider silk-silica fusion (chimeric) proteins' Proceedings of the National Academy of Sciences , vol. 103, no. 25, pp. 9428-9433. https://doi.org/10.1073/pnas.0601096103

Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. / Foo, C W P ; Patwardhan, S V ; Belton, D J ; Kitchel, B ; Anastasiades, D ; Huang, J ; Naik, R R ; Perry, C C ; Kaplan, D L .

In: Proceedings of the National Academy of Sciences , Vol. 103, No. 25, 20.06.2006, p. 9428-9433.

Research output: Contribution to journalArticle

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T1 - Novel nanocomposites from spider silk-silica fusion (chimeric) proteins

AU - Foo, C W P

AU - Patwardhan, S V

AU - Belton, D J

AU - Kitchel, B

AU - Anastasiades, D

AU - Huang, J

AU - Naik, R R

AU - Perry, C C

AU - Kaplan, D L

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KW - nanostructures

KW - silaffin

KW - biomineralization

KW - ceramics

KW - BIOACTIVE COMPOSITE-MATERIALS

KW - fibroin

KW - silicification

KW - morphogenesis

KW - crystals

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DO - 10.1073/pnas.0601096103

M3 - Article

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JO - Proceedings of the National Academy of Sciences

T2 - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

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ER -

Foo CWP, Patwardhan SV, Belton DJ, Kitchel B, Anastasiades D, Huang J et al. Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. Proceedings of the National Academy of Sciences . 2006 Jun 20;103(25):9428-9433. https://doi.org/10.1073/pnas.0601096103