Novel nanocomposites from spider silk-silica fusion (chimeric) proteins

C W P Foo, S V Patwardhan, D J Belton, B Kitchel, D Anastasiades, J Huang, R R Naik, C C Perry, D L Kaplan

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Abstract

Silica skeletal architectures in diatoms are characterized by remarkable morphological and nanostructural details. Silk proteins from spiders and silkworms form strong and intricate self-assembling fibrous biomaterials in nature. We combined the features of silk with biosilica through the design, synthesis, and characterization of a novel family of chimeric proteins for subsequent use in model materials forming reactions. The domains from the major ampullate spidroin 1 (MaSp1) protein of Nephila clavipes spider dragline silk provide control over structural and morphological details because it can be self-assembled through diverse processing methods including film casting and fiber electrospinning. Biosilica nanostructures in diatoms are formed in aqueous ambient conditions at neutral pH and low temperatures. The R5 peptide derived from the silaffin protein of Cylindrotheca fusiformis induces and regulates silica precipitation in the chimeric protein designs under similar ambient conditions. Whereas mineralization reactions performed in the presence of R5 peptide alone form silica particles with a size distribution of 0.5-10 mu m in diameter, reactions performed in the presence of the new fusion proteins generate nanocomposite materials containing silica particles with a narrower size distribution of 0.5-2 mu m in diameter. Furthermore, we demonstrate that composite morphology and structure could be regulated by controlling processing conditions to produce films and fibers. These results suggest that the chimeric protein provides new options for processing and control over silica particle sizes, important benefits for biomedical and specialty materials, particularly in light of the all aqueous processing and the nanocomposite features of these new materials.

Original languageEnglish
Pages (from-to)9428-9433
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume103
Issue number25
DOIs
Publication statusPublished - 20 Jun 2006

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Keywords

  • biomaterials
  • nanostructures
  • silaffin
  • biomineralization
  • ceramics
  • BIOACTIVE COMPOSITE-MATERIALS
  • fibroin
  • silicification
  • morphogenesis
  • crystals

Cite this

Foo, C. W. P., Patwardhan, S. V., Belton, D. J., Kitchel, B., Anastasiades, D., Huang, J., ... Kaplan, D. L. (2006). Novel nanocomposites from spider silk-silica fusion (chimeric) proteins. Proceedings of the National Academy of Sciences , 103(25), 9428-9433. https://doi.org/10.1073/pnas.0601096103