Novel nano-biomaterials for inhibition of respiratory syncytial virus

S. Joshi, D. Kirby, Y. Perrie, S. R. Singh

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

1 Citation (Scopus)

Abstract

The liposomes were prepared using the conventional thin film hydration method with and without peptide RF-482. The loading of RF-482 was determined using BCA assay and the RSV inhibition was studied using immune-fluorescence imaging, plaque assay and PCR. There was little size change observed after loading RF-482. However, this change was not significant. It was observed that 77.3 % (n=3, ±2.2 %) protein was found non-encapsulated, hence it can be concluded that 22.7 % of peptide RF-482 was encapsulated. Plaque assay confirms above 60 % inhibition with just peptide RF-482 and liposomes. However, with peptide loaded liposomes more than 70 % inhibition was observed. Screening of RSV-F gene amplicon and comparison of viral gene amplicon and peptide, liposomes as well as functionalised liposomes along with water as negative control confirms the competitive inhibition of RSV by RF-482 as well as liposomes with and without peptide. Overall, it was found that similar to GNPs and FGNPs (Tiwari et al., 2014), empty liposomes as well as liposomes loaded with RF-482 can inhibit RSV fusion to HEP-2 cells and thereby save HEP-2 cells from infection. Immunofluorescence imaging confirms the inhibitory effect of liposomes with and without peptide RF-482.

LanguageEnglish
Title of host publicationBiotech, Biomaterials and Biomedical
Subtitle of host publicationTechConnect Briefs 2017
Place of PublicationCalifornia
Pages75-78
Number of pages4
Publication statusPublished - 14 May 2017
Event11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States
Duration: 14 May 201717 May 2017

Conference

Conference11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
CountryUnited States
CityWashington
Period14/05/1717/05/17

Fingerprint

Respiratory Syncytial Viruses
Liposomes
Biocompatible Materials
Viruses
Biomaterials
Peptides
Assays
Genes
Imaging techniques
Viral Genes
Optical Imaging
Hydration
Fluorescent Antibody Technique
Screening
Fusion reactions
Fluorescence
Proteins
Thin films
Polymerase Chain Reaction
Water

Keywords

  • nano-biomaterial
  • liposome
  • respiratory syncytial virus

Cite this

Joshi, S., Kirby, D., Perrie, Y., & Singh, S. R. (2017). Novel nano-biomaterials for inhibition of respiratory syncytial virus. In Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017 (pp. 75-78). California.
Joshi, S. ; Kirby, D. ; Perrie, Y. ; Singh, S. R. / Novel nano-biomaterials for inhibition of respiratory syncytial virus. Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017. California, 2017. pp. 75-78
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abstract = "The liposomes were prepared using the conventional thin film hydration method with and without peptide RF-482. The loading of RF-482 was determined using BCA assay and the RSV inhibition was studied using immune-fluorescence imaging, plaque assay and PCR. There was little size change observed after loading RF-482. However, this change was not significant. It was observed that 77.3 {\%} (n=3, ±2.2 {\%}) protein was found non-encapsulated, hence it can be concluded that 22.7 {\%} of peptide RF-482 was encapsulated. Plaque assay confirms above 60 {\%} inhibition with just peptide RF-482 and liposomes. However, with peptide loaded liposomes more than 70 {\%} inhibition was observed. Screening of RSV-F gene amplicon and comparison of viral gene amplicon and peptide, liposomes as well as functionalised liposomes along with water as negative control confirms the competitive inhibition of RSV by RF-482 as well as liposomes with and without peptide. Overall, it was found that similar to GNPs and FGNPs (Tiwari et al., 2014), empty liposomes as well as liposomes loaded with RF-482 can inhibit RSV fusion to HEP-2 cells and thereby save HEP-2 cells from infection. Immunofluorescence imaging confirms the inhibitory effect of liposomes with and without peptide RF-482.",
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Joshi, S, Kirby, D, Perrie, Y & Singh, SR 2017, Novel nano-biomaterials for inhibition of respiratory syncytial virus. in Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017. California, pp. 75-78, 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference, Washington, United States, 14/05/17.

Novel nano-biomaterials for inhibition of respiratory syncytial virus. / Joshi, S.; Kirby, D.; Perrie, Y.; Singh, S. R.

Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017. California, 2017. p. 75-78.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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T1 - Novel nano-biomaterials for inhibition of respiratory syncytial virus

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N2 - The liposomes were prepared using the conventional thin film hydration method with and without peptide RF-482. The loading of RF-482 was determined using BCA assay and the RSV inhibition was studied using immune-fluorescence imaging, plaque assay and PCR. There was little size change observed after loading RF-482. However, this change was not significant. It was observed that 77.3 % (n=3, ±2.2 %) protein was found non-encapsulated, hence it can be concluded that 22.7 % of peptide RF-482 was encapsulated. Plaque assay confirms above 60 % inhibition with just peptide RF-482 and liposomes. However, with peptide loaded liposomes more than 70 % inhibition was observed. Screening of RSV-F gene amplicon and comparison of viral gene amplicon and peptide, liposomes as well as functionalised liposomes along with water as negative control confirms the competitive inhibition of RSV by RF-482 as well as liposomes with and without peptide. Overall, it was found that similar to GNPs and FGNPs (Tiwari et al., 2014), empty liposomes as well as liposomes loaded with RF-482 can inhibit RSV fusion to HEP-2 cells and thereby save HEP-2 cells from infection. Immunofluorescence imaging confirms the inhibitory effect of liposomes with and without peptide RF-482.

AB - The liposomes were prepared using the conventional thin film hydration method with and without peptide RF-482. The loading of RF-482 was determined using BCA assay and the RSV inhibition was studied using immune-fluorescence imaging, plaque assay and PCR. There was little size change observed after loading RF-482. However, this change was not significant. It was observed that 77.3 % (n=3, ±2.2 %) protein was found non-encapsulated, hence it can be concluded that 22.7 % of peptide RF-482 was encapsulated. Plaque assay confirms above 60 % inhibition with just peptide RF-482 and liposomes. However, with peptide loaded liposomes more than 70 % inhibition was observed. Screening of RSV-F gene amplicon and comparison of viral gene amplicon and peptide, liposomes as well as functionalised liposomes along with water as negative control confirms the competitive inhibition of RSV by RF-482 as well as liposomes with and without peptide. Overall, it was found that similar to GNPs and FGNPs (Tiwari et al., 2014), empty liposomes as well as liposomes loaded with RF-482 can inhibit RSV fusion to HEP-2 cells and thereby save HEP-2 cells from infection. Immunofluorescence imaging confirms the inhibitory effect of liposomes with and without peptide RF-482.

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Joshi S, Kirby D, Perrie Y, Singh SR. Novel nano-biomaterials for inhibition of respiratory syncytial virus. In Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017. California. 2017. p. 75-78