Novel enrofloxacin imprinted polymer applied to the solid-phase extraction of fluorinated quinolones from urine and tissue samples

Ester Caro, Rosa M. Marcé, Peter A.G. Cormack, David C. Sherrington, Francesc Borrull

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

A new molecularly imprinted polymer, prepared following a non-covalent approach, was synthesised using enrofloxacin as a template molecule. The imprinting effect of the polymer was verified by chromatographic evaluation and, interestingly, this evaluation also revealed that the imprinted polymer showed a high degree of cross-reactivity for ciprofloxacin, the major metabolite of enrofloxacin. The molecularly imprinted polymer was then applied as a selective sorbent in a two-step solid-phase extraction method focussing upon complex biological matrices, specifically human urine and pig liver. This two-step solid-phase extraction protocol, in which a commercial Oasis HLB cartridge and a molecularly imprinted solid-phase extraction cartridge were combined, allowed enrofloxacin and ciprofloxacin to be determined by liquid chromatography coupled to a UV detector at levels below the maximum residue limits established by the European Union. The quantification and detection limits in tissue samples of enrofloxacin and ciprofloxacin were established at 50 μg kg−1 and 30 μg kg−1, respectively.
LanguageEnglish
Pages145-151
Number of pages7
JournalAnalytica Chimica Acta
Volume562
Issue number2
DOIs
Publication statusPublished - 15 Mar 2006

Fingerprint

Quinolones
Solid Phase Extraction
urine
Polymers
Ciprofloxacin
polymer
Urine
Tissue
Ultraviolet detectors
imprinting
Liquid chromatography
oasis
European Union
Metabolites
Sorbents
extraction method
Liquid Chromatography
pig
Liver
Limit of Detection

Keywords

  • molecular imprinting
  • solid-phase extraction
  • fluorinated quinolones
  • urine samples
  • tissue samples

Cite this

Caro, Ester ; Marcé, Rosa M. ; Cormack, Peter A.G. ; Sherrington, David C. ; Borrull, Francesc. / Novel enrofloxacin imprinted polymer applied to the solid-phase extraction of fluorinated quinolones from urine and tissue samples. In: Analytica Chimica Acta. 2006 ; Vol. 562, No. 2. pp. 145-151.
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Novel enrofloxacin imprinted polymer applied to the solid-phase extraction of fluorinated quinolones from urine and tissue samples. / Caro, Ester; Marcé, Rosa M.; Cormack, Peter A.G.; Sherrington, David C.; Borrull, Francesc.

In: Analytica Chimica Acta, Vol. 562, No. 2, 15.03.2006, p. 145-151.

Research output: Contribution to journalArticle

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