Notch signaling regulates follicular helper T cell differentiation

Floriane Auderset, Steffen Schuster, Nicolas Fasnacht, Manuel Coutaz, Mélanie Charmoy, Ute Koch, Stéphanie Favre, Anne Wilson, François Trottein, James Alexander, Sanjiv A Luther, H Robson Macdonald, Freddy Radtke, Fabienne Tacchini-Cottier

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Follicular helper T (TFH) cells are specialized in providing help for B cell differentiation and Ab secretion. Several positive and negative regulators of TFH cell differentiation have been described but their control is not fully understood. In this study, we show that Notch signaling in T cells is a major player in the development and function of TFH cells. T cell-specific gene ablation of Notch1 and Notch2 impaired differentiation of TFH cells in draining lymph nodes of mice immunized with T-dependent Ags or infected with parasites. Impaired TFH cell differentiation correlated with deficient germinal center development and the absence of high-affinity Abs. The impact of loss of Notch on TFH cell differentiation was largely independent of its effect on IL-4. These results show a previously unknown role for Notch in the regulation of TFH cell differentiation and function with implications for the control of this T cell population.
LanguageEnglish
Pages2344-2350
Number of pages7
JournalJournal of Immunology
Volume191
Issue number5
DOIs
Publication statusPublished - 1 Sep 2013

Fingerprint

Helper-Inducer T-Lymphocytes
Cell Differentiation
T-Lymphocytes
Germinal Center
Interleukin-4
Parasites
B-Lymphocytes
Lymph Nodes
Population
Genes

Keywords

  • follicular helper T cells
  • notch signaling

Cite this

Auderset, F., Schuster, S., Fasnacht, N., Coutaz, M., Charmoy, M., Koch, U., ... Tacchini-Cottier, F. (2013). Notch signaling regulates follicular helper T cell differentiation. Journal of Immunology , 191(5), 2344-2350. https://doi.org/10.4049/jimmunol.1300643
Auderset, Floriane ; Schuster, Steffen ; Fasnacht, Nicolas ; Coutaz, Manuel ; Charmoy, Mélanie ; Koch, Ute ; Favre, Stéphanie ; Wilson, Anne ; Trottein, François ; Alexander, James ; Luther, Sanjiv A ; Macdonald, H Robson ; Radtke, Freddy ; Tacchini-Cottier, Fabienne. / Notch signaling regulates follicular helper T cell differentiation. In: Journal of Immunology . 2013 ; Vol. 191, No. 5. pp. 2344-2350.
@article{87b16f89731d4401956e9f77ee64f6ea,
title = "Notch signaling regulates follicular helper T cell differentiation",
abstract = "Follicular helper T (TFH) cells are specialized in providing help for B cell differentiation and Ab secretion. Several positive and negative regulators of TFH cell differentiation have been described but their control is not fully understood. In this study, we show that Notch signaling in T cells is a major player in the development and function of TFH cells. T cell-specific gene ablation of Notch1 and Notch2 impaired differentiation of TFH cells in draining lymph nodes of mice immunized with T-dependent Ags or infected with parasites. Impaired TFH cell differentiation correlated with deficient germinal center development and the absence of high-affinity Abs. The impact of loss of Notch on TFH cell differentiation was largely independent of its effect on IL-4. These results show a previously unknown role for Notch in the regulation of TFH cell differentiation and function with implications for the control of this T cell population.",
keywords = "follicular helper T cells, notch signaling",
author = "Floriane Auderset and Steffen Schuster and Nicolas Fasnacht and Manuel Coutaz and M{\'e}lanie Charmoy and Ute Koch and St{\'e}phanie Favre and Anne Wilson and Fran{\cc}ois Trottein and James Alexander and Luther, {Sanjiv A} and Macdonald, {H Robson} and Freddy Radtke and Fabienne Tacchini-Cottier",
year = "2013",
month = "9",
day = "1",
doi = "10.4049/jimmunol.1300643",
language = "English",
volume = "191",
pages = "2344--2350",
journal = "Journal of Immunology",
issn = "0022-1767",
number = "5",

}

Auderset, F, Schuster, S, Fasnacht, N, Coutaz, M, Charmoy, M, Koch, U, Favre, S, Wilson, A, Trottein, F, Alexander, J, Luther, SA, Macdonald, HR, Radtke, F & Tacchini-Cottier, F 2013, 'Notch signaling regulates follicular helper T cell differentiation' Journal of Immunology , vol. 191, no. 5, pp. 2344-2350. https://doi.org/10.4049/jimmunol.1300643

Notch signaling regulates follicular helper T cell differentiation. / Auderset, Floriane; Schuster, Steffen; Fasnacht, Nicolas; Coutaz, Manuel; Charmoy, Mélanie; Koch, Ute; Favre, Stéphanie; Wilson, Anne; Trottein, François; Alexander, James; Luther, Sanjiv A; Macdonald, H Robson; Radtke, Freddy; Tacchini-Cottier, Fabienne.

In: Journal of Immunology , Vol. 191, No. 5, 01.09.2013, p. 2344-2350.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Notch signaling regulates follicular helper T cell differentiation

AU - Auderset, Floriane

AU - Schuster, Steffen

AU - Fasnacht, Nicolas

AU - Coutaz, Manuel

AU - Charmoy, Mélanie

AU - Koch, Ute

AU - Favre, Stéphanie

AU - Wilson, Anne

AU - Trottein, François

AU - Alexander, James

AU - Luther, Sanjiv A

AU - Macdonald, H Robson

AU - Radtke, Freddy

AU - Tacchini-Cottier, Fabienne

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Follicular helper T (TFH) cells are specialized in providing help for B cell differentiation and Ab secretion. Several positive and negative regulators of TFH cell differentiation have been described but their control is not fully understood. In this study, we show that Notch signaling in T cells is a major player in the development and function of TFH cells. T cell-specific gene ablation of Notch1 and Notch2 impaired differentiation of TFH cells in draining lymph nodes of mice immunized with T-dependent Ags or infected with parasites. Impaired TFH cell differentiation correlated with deficient germinal center development and the absence of high-affinity Abs. The impact of loss of Notch on TFH cell differentiation was largely independent of its effect on IL-4. These results show a previously unknown role for Notch in the regulation of TFH cell differentiation and function with implications for the control of this T cell population.

AB - Follicular helper T (TFH) cells are specialized in providing help for B cell differentiation and Ab secretion. Several positive and negative regulators of TFH cell differentiation have been described but their control is not fully understood. In this study, we show that Notch signaling in T cells is a major player in the development and function of TFH cells. T cell-specific gene ablation of Notch1 and Notch2 impaired differentiation of TFH cells in draining lymph nodes of mice immunized with T-dependent Ags or infected with parasites. Impaired TFH cell differentiation correlated with deficient germinal center development and the absence of high-affinity Abs. The impact of loss of Notch on TFH cell differentiation was largely independent of its effect on IL-4. These results show a previously unknown role for Notch in the regulation of TFH cell differentiation and function with implications for the control of this T cell population.

KW - follicular helper T cells

KW - notch signaling

UR - http://www.scopus.com/inward/record.url?scp=84883396890&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.1300643

DO - 10.4049/jimmunol.1300643

M3 - Article

VL - 191

SP - 2344

EP - 2350

JO - Journal of Immunology

T2 - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -

Auderset F, Schuster S, Fasnacht N, Coutaz M, Charmoy M, Koch U et al. Notch signaling regulates follicular helper T cell differentiation. Journal of Immunology . 2013 Sep 1;191(5):2344-2350. https://doi.org/10.4049/jimmunol.1300643