Non-invasive analysis in micro-reactors using Raman spectrometry with a specially designed probe

Sergey Mozharov, A. Nordon, John Girkin, D. Littlejohn

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)
443 Downloads (Pure)


An optical interface has been designed to maximise the sensitivity and spatial resolution required when Raman spectrometry is used to monitor a reaction in a micro-reactor, revealing advantages over a conventional commercial probe. A miniature aspheric lens was shown to be better than microscope objectives to focus the probing laser beam on to the sample. The diameters of the exciting and collection optical fibres were also shown to have a significant influence on sensitivity and the signal to background ratio, with 62.5 m diameter 0.28 numerical aperture (NA) fibres found to be best for analysis of liquids in the 150 m-deep channel in the micro-reactor used. With a spectral measurement time of 2 s, it was shown that the probe could monitor the progress of an esterification reaction in real time and quickly optimise the reagent flow rates. The fast response time revealed features related to short-term pump instabilities and micro-reactor rheology effects that would not have been identified without rapid real-time measurements.
Original languageEnglish
Pages (from-to)2101-2107
Number of pages7
JournalLab on a Chip
Issue number16
Early online date14 Jun 2010
Publication statusPublished - 21 Aug 2010


  • non-invasive analysis
  • probe
  • Raman spectrometry
  • micro-reactors


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