Abstract
| Language | English |
|---|---|
| Pages | 2620-2624 |
| Number of pages | 4 |
| Journal | Journal of Physics D: Applied Physics |
| Volume | 38 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - 7 Aug 2005 |
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Keywords
- signal amplitude fluctuations
- white-light supercontinuum
- CLSM excitation sources
- guinea pig detrusor
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Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy. / McConnell, G.
In: Journal of Physics D: Applied Physics, Vol. 38, No. 15, 07.08.2005, p. 2620-2624.Research output: Contribution to journal › Article
TY - JOUR
T1 - Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy
AU - McConnell, G.
PY - 2005/8/7
Y1 - 2005/8/7
N2 - Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.
AB - Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.
KW - signal amplitude fluctuations
KW - white-light supercontinuum
KW - CLSM excitation sources
KW - guinea pig detrusor
UR - http://dx.doi.org/10.1088/0022-3727/38/15/013
U2 - 10.1088/0022-3727/38/15/013
DO - 10.1088/0022-3727/38/15/013
M3 - Article
VL - 38
SP - 2620
EP - 2624
JO - Journal of Physics D: Applied Physics
T2 - Journal of Physics D: Applied Physics
JF - Journal of Physics D: Applied Physics
SN - 0022-3727
IS - 15
ER -