Abstract
Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy
(CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM
excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system
flexibility.
Original language | English |
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Pages (from-to) | 2620-2624 |
Number of pages | 4 |
Journal | Journal of Physics D: Applied Physics |
Volume | 38 |
Issue number | 15 |
DOIs | |
Publication status | Published - 7 Aug 2005 |
Keywords
- signal amplitude fluctuations
- white-light supercontinuum
- CLSM excitation sources
- guinea pig detrusor