Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy

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Abstract

Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.
LanguageEnglish
Pages2620-2624
Number of pages4
JournalJournal of Physics D: Applied Physics
Volume38
Issue number15
DOIs
Publication statusPublished - 7 Aug 2005

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Fluorescence microscopy
Light sources
light sources
microscopy
Scanning
Wavelength
fluorescence
scanning
Lasers
sapphire
Microscopic examination
wavelengths
Aluminum Oxide
lasers
guinea pigs
Sapphire
low noise
flexibility
pixels
White noise

Keywords

  • signal amplitude fluctuations
  • white-light supercontinuum
  • CLSM excitation sources
  • guinea pig detrusor

Cite this

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title = "Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy",
abstract = "Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98{\%}, 95{\%} and 94{\%} were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.",
keywords = "signal amplitude fluctuations, white-light supercontinuum, CLSM excitation sources, guinea pig detrusor",
author = "G. McConnell",
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AU - McConnell, G.

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AB - Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.

KW - signal amplitude fluctuations

KW - white-light supercontinuum

KW - CLSM excitation sources

KW - guinea pig detrusor

UR - http://dx.doi.org/10.1088/0022-3727/38/15/013

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DO - 10.1088/0022-3727/38/15/013

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