NMR spectroscopy methods in metabolic phenotyping

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

NMR spectroscopy is an established, versatile, widely applicable analytical technique forming one of the cornerstone methods for metabolic profiling, metabonomics, metabolomics, and metabolic phenotyping applied to complex biofluid and tissue samples. From sample storage and preparation conditions through basic one-dimensional proton and two-dimensional proton only and proton-carbon correlated NMR techniques, this chapter moves on to consider the quest for ever more advanced NMR methods to improve data resolution, increase analyte detection limits, and robustly quantify components within complex biofluid and tissue matrices. The work is a guide to the established state-of-the-art in bioanalytical NMR methods, and through its course addresses some pitfalls to be aware of as well as providing a window through which the future potential shape of the field may be considered.
Original languageEnglish
Title of host publicationThe Handbook of Metabolic Phenotyping
EditorsJohn Lindon, Jeremy Nicholson, Elaine Holmes
Place of PublicationCambridge, MA
Chapter2
Pages53-96
Number of pages44
DOIs
Publication statusPublished - 5 Oct 2018

Keywords

  • NMR spectroscopy
  • metabolic phenotyping
  • metabolimics
  • metabonomics

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  • Equipment

    NMR Facility

    John Parkinson (Manager)

    Pure And Applied Chemistry

    Facility/equipment: Facility

  • Cite this

    Parkinson, J. A. (2018). NMR spectroscopy methods in metabolic phenotyping. In J. Lindon, J. Nicholson, & E. Holmes (Eds.), The Handbook of Metabolic Phenotyping (pp. 53-96). https://doi.org/10.1016/B978-0-12-812293-8.00002-5