Nitric oxide synthases in infants and children with pulmonary hypertension and congenital heart disease

Thomas Hoehn, Brigitte Stiller, Allan R. McPhaden, Roger M. Wadsworth

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nitric oxide is an important regulator of vascular tone in the pulmonary circulation. Surgical correction of congenital heart disease limits pulmonary hypertension to a brief period. The study has measured expression of endothelial (eNOS), inducible (iNOS), and neuronal nitric oxide synthase (nNOS) in the lungs from biopsies of infants with pulmonary hypertension secondary to cardiac abnormalities (n = 26), compared to a control group who did not have pulmonary or cardiac disease (n = 8). eNOS, iNOS and nNOS were identified by immunohistochemistry and quantified in specific cell types. Significant increases of eNOS and iNOS staining were found in pulmonary vascular endothelial cells of patients with congenital heart disease compared to control infants. These changes were confined to endothelial cells and not present in other cell types. Patients who strongly expressed eNOS also had strong expression of iNOS.
Upregulation of eNOS and iNOS occurs at an early stage of pulmonary hypertension, and may be a compensatory mechanism limiting the rise in pulmonary artery pressure.

LanguageEnglish
Article number110
Number of pages9
JournalRespiratory Research
Volume10
DOIs
Publication statusPublished - 13 Nov 2009

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Pulmonary Hypertension
Nitric Oxide Synthase
Heart Diseases
Nitric Oxide Synthase Type I
Endothelial Cells
Lung
Pulmonary Circulation
Nitric Oxide Synthase Type II
Pulmonary Artery
Lung Diseases
Blood Vessels
Nitric Oxide
Up-Regulation
Immunohistochemistry
Staining and Labeling
Biopsy
Pressure
Control Groups

Keywords

  • diaphragmatic-hernia
  • endothelial-cells
  • chronic hypoxia
  • expression
  • gene
  • rat
  • therapy
  • circulation
  • decreases
  • newborns

Cite this

Hoehn, Thomas ; Stiller, Brigitte ; McPhaden, Allan R. ; Wadsworth, Roger M. / Nitric oxide synthases in infants and children with pulmonary hypertension and congenital heart disease. In: Respiratory Research. 2009 ; Vol. 10.
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Nitric oxide synthases in infants and children with pulmonary hypertension and congenital heart disease. / Hoehn, Thomas; Stiller, Brigitte; McPhaden, Allan R.; Wadsworth, Roger M.

In: Respiratory Research, Vol. 10, 110, 13.11.2009.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Hoehn, Thomas

AU - Stiller, Brigitte

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AU - Wadsworth, Roger M.

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AB - Nitric oxide is an important regulator of vascular tone in the pulmonary circulation. Surgical correction of congenital heart disease limits pulmonary hypertension to a brief period. The study has measured expression of endothelial (eNOS), inducible (iNOS), and neuronal nitric oxide synthase (nNOS) in the lungs from biopsies of infants with pulmonary hypertension secondary to cardiac abnormalities (n = 26), compared to a control group who did not have pulmonary or cardiac disease (n = 8). eNOS, iNOS and nNOS were identified by immunohistochemistry and quantified in specific cell types. Significant increases of eNOS and iNOS staining were found in pulmonary vascular endothelial cells of patients with congenital heart disease compared to control infants. These changes were confined to endothelial cells and not present in other cell types. Patients who strongly expressed eNOS also had strong expression of iNOS.Upregulation of eNOS and iNOS occurs at an early stage of pulmonary hypertension, and may be a compensatory mechanism limiting the rise in pulmonary artery pressure.

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KW - chronic hypoxia

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KW - gene

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KW - therapy

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