New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition

Karl Payne, Mark White, Karl Fisher, Basile Khara, Samuel Bailey, David Parker, Nicholas Rattray, Drupad K Trivedi, Royston Goodacre, Rebecca Beveridge, Perdita Barran, Stephen Rigby, Nigel Scrutton, Sam Hay, David Leys

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The bacterial ubiD and ubiX or the homologous fungal fdc1 and pad1 genes have been implicated in the non-oxidative reversible decarboxylation of aromatic substrates, and play a pivotal role in bacterial ubiquinone (also known as coenzyme Q) biosynthesis1,2,3 or microbial biodegradation of aromatic compounds4,5,6, respectively. Despite biochemical studies on individual gene products, the composition and cofactor requirement of the enzyme responsible for in vivo decarboxylase activity remained unclear7,8,9. Here we show that Fdc1 is solely responsible for the reversible decarboxylase activity, and that it requires a new type of cofactor: a prenylated flavin synthesized by the associated UbiX/Pad110. Atomic resolution crystal structures reveal that two distinct isomers of the oxidized cofactor can be observed, an isoalloxazine N5-iminium adduct and a N5 secondary ketimine species with markedly altered ring structure, both having azomethine ylide character. Substrate binding positions the dipolarophile enoic acid group directly above the azomethine ylide group. The structure of a covalent inhibitor–cofactor adduct suggests that 1,3-dipolar cycloaddition chemistry supports reversible decarboxylation in these enzymes. Although 1,3-dipolar cycloaddition is commonly used in organic chemistry11,12, we propose that this presents the first example, to our knowledge, of an enzymatic 1,3-dipolar cycloaddition reaction. Our model for Fdc1/UbiD catalysis offers new routes in alkene hydrocarbon production or aryl (de)carboxylation.
LanguageEnglish
Pages497-501
Number of pages5
JournalNature
Volume522
DOIs
Publication statusPublished - 25 Jun 2015

Fingerprint

Decarboxylation
Cycloaddition Reaction
Ubiquinone
Carboxy-Lyases
Acids
Coenzymes
Alkenes
Hydrocarbons
Catalysis
Genes
Enzymes
azomethine

Keywords

  • ubiquinone
  • coenzyme Q
  • microbial biodegradation

Cite this

Payne, K., White, M., Fisher, K., Khara, B., Bailey, S., Parker, D., ... Leys, D. (2015). New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition. Nature, 522, 497-501. https://doi.org/10.1038/nature14560
Payne, Karl ; White, Mark ; Fisher, Karl ; Khara, Basile ; Bailey, Samuel ; Parker, David ; Rattray, Nicholas ; Trivedi, Drupad K ; Goodacre, Royston ; Beveridge, Rebecca ; Barran, Perdita ; Rigby, Stephen ; Scrutton, Nigel ; Hay, Sam ; Leys, David. / New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition. In: Nature. 2015 ; Vol. 522. pp. 497-501.
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Payne, K, White, M, Fisher, K, Khara, B, Bailey, S, Parker, D, Rattray, N, Trivedi, DK, Goodacre, R, Beveridge, R, Barran, P, Rigby, S, Scrutton, N, Hay, S & Leys, D 2015, 'New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition' Nature, vol. 522, pp. 497-501. https://doi.org/10.1038/nature14560

New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition. / Payne, Karl; White, Mark; Fisher, Karl; Khara, Basile; Bailey, Samuel; Parker, David; Rattray, Nicholas; Trivedi, Drupad K; Goodacre, Royston; Beveridge, Rebecca; Barran, Perdita; Rigby, Stephen; Scrutton, Nigel; Hay, Sam; Leys, David.

In: Nature, Vol. 522, 25.06.2015, p. 497-501.

Research output: Contribution to journalArticle

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T1 - New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition

AU - Payne, Karl

AU - White, Mark

AU - Fisher, Karl

AU - Khara, Basile

AU - Bailey, Samuel

AU - Parker, David

AU - Rattray, Nicholas

AU - Trivedi, Drupad K

AU - Goodacre, Royston

AU - Beveridge, Rebecca

AU - Barran, Perdita

AU - Rigby, Stephen

AU - Scrutton, Nigel

AU - Hay, Sam

AU - Leys, David

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Payne K, White M, Fisher K, Khara B, Bailey S, Parker D et al. New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition. Nature. 2015 Jun 25;522:497-501. https://doi.org/10.1038/nature14560