Abstract
Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.
| Language | English |
|---|---|
| Number of pages | 9 |
| Journal | AMB Express |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 24 Jan 2019 |
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Keywords
- Actinokineospora
- actinomycetes
- antitrypanosomal
- elicitation
- fridamycin
- sponges
Cite this
}
New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov. / Tawfike, Ahmed; Attia, Eman Zekry; Desoukey, Samar Yehia; Hajjar, Dina; Makki, Arwa A.; Schupp, Peter J.; Edrada-Ebel, RuAngelie; Abdelmohsen, Usama Ramadan.
In: AMB Express, Vol. 9, No. 1, 24.01.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov.
AU - Tawfike, Ahmed
AU - Attia, Eman Zekry
AU - Desoukey, Samar Yehia
AU - Hajjar, Dina
AU - Makki, Arwa A.
AU - Schupp, Peter J.
AU - Edrada-Ebel, RuAngelie
AU - Abdelmohsen, Usama Ramadan
PY - 2019/1/24
Y1 - 2019/1/24
N2 - Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.
AB - Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.
KW - Actinokineospora
KW - actinomycetes
KW - antitrypanosomal
KW - elicitation
KW - fridamycin
KW - sponges
UR - http://www.scopus.com/inward/record.url?scp=85060637606&partnerID=8YFLogxK
UR - https://amb-express.springeropen.com/
U2 - 10.1186/s13568-018-0730-0
DO - 10.1186/s13568-018-0730-0
M3 - Article
VL - 9
JO - Optics Express
T2 - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 1
ER -