New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov.

Ahmed Tawfike, Eman Zekry Attia, Samar Yehia Desoukey, Dina Hajjar, Arwa A. Makki, Peter J. Schupp, RuAngelie Edrada-Ebel, Usama Ramadan Abdelmohsen

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Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.

Original languageEnglish
Number of pages9
JournalAMB Express
Issue number1
Publication statusPublished - 24 Jan 2019


  • Actinokineospora
  • actinomycetes
  • antitrypanosomal
  • elicitation
  • fridamycin
  • sponges

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