New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov.

Ahmed Tawfike, Eman Zekry Attia, Samar Yehia Desoukey, Dina Hajjar, Arwa A. Makki, Peter J. Schupp, RuAngelie Edrada-Ebel, Usama Ramadan Abdelmohsen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Several approaches have been dedicated to activate the cryptic gene clusters in the genomes of actinomycetes for the targeted discovery of new fascinating biomedical lead structures. In the current study, N-acetylglucosamine was used to maximize the chemical diversity of sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR–ESI–MS was employed for dereplication study and orthogonal partial least square-discriminant analysis was applied to evaluate the HR–ESI–MS data of the different fractions. As a result, two new fridamycins H (1) and I (2), along with three known compounds actinosporin C (3), D (4), and G (5) were isolated from the solid culture of sponge-associated actinomycete Actinokineospora spheciospongiae sp. nov., elicited with N-acetylglucosamine. Characterization of the isolated compounds was pursued using mass spectrometry and NMR spectral data. Fridamycin H (1) exhibited significant growth inhibitory activity towards Trypanosoma brucei strain TC221. These results highlight the potential of elicitation in sponge-associated actinomycetes as an effective strategy for the discovery of new anti-infective natural products.

LanguageEnglish
Number of pages9
JournalAMB Express
Volume9
Issue number1
DOIs
Publication statusPublished - 24 Jan 2019

Fingerprint

actinomycetes
Actinobacteria
metabolites
Porifera
activity (biology)
bacteria
Bacteria
Acetylglucosamine
Trypanosoma brucei brucei
genome
Discriminant Analysis
Multigene Family
Biological Products
Least-Squares Analysis
genes
Mass Spectrometry
mass spectroscopy
Genome
nuclear magnetic resonance
products

Keywords

  • Actinokineospora
  • actinomycetes
  • antitrypanosomal
  • elicitation
  • fridamycin
  • sponges

Cite this

Tawfike, A., Attia, E. Z., Desoukey, S. Y., Hajjar, D., Makki, A. A., Schupp, P. J., ... Abdelmohsen, U. R. (2019). New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov. AMB Express, 9(1). https://doi.org/10.1186/s13568-018-0730-0
Tawfike, Ahmed ; Attia, Eman Zekry ; Desoukey, Samar Yehia ; Hajjar, Dina ; Makki, Arwa A. ; Schupp, Peter J. ; Edrada-Ebel, RuAngelie ; Abdelmohsen, Usama Ramadan. / New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov. In: AMB Express. 2019 ; Vol. 9, No. 1.
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New bioactive metabolites from the elicited marine sponge-derived bacterium Actinokineospora spheciospongiae sp. nov. / Tawfike, Ahmed; Attia, Eman Zekry; Desoukey, Samar Yehia; Hajjar, Dina; Makki, Arwa A.; Schupp, Peter J.; Edrada-Ebel, RuAngelie; Abdelmohsen, Usama Ramadan.

In: AMB Express, Vol. 9, No. 1, 24.01.2019.

Research output: Contribution to journalArticle

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