New antifouling platform characterized by single-molecule imaging

Ji Young Ryu, In Taek Song, K. H. Aaron Lau, Phillip B. Messersmith, Tae-Young Yoon, Haeshin Lee

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)
82 Downloads (Pure)


Antifouling surfaces have been widely studied for their importance in medical devices and industry. Antifouling surfaces mostly achieved by methoxy-poly(ethylene glycol) (mPEG) have shown biomolecular adsorption less than 1 ng/cm2 which was measured by surface analytical tools such as surface plasmon resonance (SPR) spectroscopy, quartz crystal microbalance (QCM), or optical waveguide lightmode (OWL) spectroscopy. Herein, we utilize a single-molecule imaging technique (i.e., an ultimate resolution) to study antifouling properties of functionalized surfaces. We found that about 600 immunoglobulin G (IgG) molecules are adsorbed. This result corresponds to ∼5 pg/cm2 adsorption, which is far below amount for the detection limit of the conventional tools. Furthermore, we developed a new antifouling platform that exhibits improved antifouling performance that shows only 78 IgG molecules adsorbed (∼0.5 pg/cm2). The antifouling platform consists of forming 1 nm TiO2 thin layer, on which peptidomimetic antifouling polymer (PMAP) is robustly anchored. The unprecedented antifouling performance can potentially revolutionize a variety of research fields such as single-molecule imaging, medical devices, biosensors, and others.

Original languageEnglish
Pages (from-to)3553-3558
Number of pages6
JournalACS Applied Materials and Interfaces
Issue number5
Publication statusPublished - 6 Feb 2014


  • antifouling
  • catecholamine
  • polypeptoid
  • single-molecule imaging


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