Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani

E. McFarlane, C. Perez, M. Charmoy, C. Allenbach, K.C. Carter, J. Alexander, F. Tacchini-Cottier

Research output: Contribution to journalArticle

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Abstract

Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The role of neutrophils during the first weeks post-infection with Leishmania donovani was investigated in this study. When L. donovani-infected mice were selectively depleted of neutrophils with the NIMP-R14 monoclonal antibody, a significant increase in parasite numbers was observed in the spleen and bone marrow and to a lesser extent in the liver. Increased susceptibility was associated with enhanced splenomegally, a delay in the maturation of hepatic granulomas, and a decrease in inducible nitric oxide synthase expression within granulomas. In the spleen, neutrophil depletion was associated with a significant increase in interleukin 4 (IL-4) and IL-10 levels and reduced gamma interferon secretion by CD4+ and CD8+ T cells. Increased production of serum IL-4 and IL-10 and higher levels of Leishmania-specific immunoglobulin G1 (IgG1) versus IgG2a revealed the preferential induction of Th2 responses in neutrophil-depleted mice. Altogether, these data suggest a critical role for neutrophils in the early protective response against L. donovani, both as effector cells involved in the killing of the parasites and as significant players influencing the development of a protective Th1 immune response.
LanguageEnglish
Pages532-541
Number of pages10
JournalInfection and Immunity
Volume76
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Leishmania donovani
Neutrophils
Infection
Granuloma
Interleukin-4
Interleukin-10
Parasites
Spleen
Leishmania
Liver
Nitric Oxide Synthase Type II
Interferon-gamma
Immunoglobulins
Bone Marrow
Monoclonal Antibodies
T-Lymphocytes
Serum

Keywords

  • neutrophils
  • immune response
  • infection
  • Leishmania donovani

Cite this

McFarlane, E. ; Perez, C. ; Charmoy, M. ; Allenbach, C. ; Carter, K.C. ; Alexander, J. ; Tacchini-Cottier, F. / Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani. In: Infection and Immunity. 2008 ; Vol. 76, No. 2. pp. 532-541.
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Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani. / McFarlane, E.; Perez, C.; Charmoy, M.; Allenbach, C.; Carter, K.C.; Alexander, J.; Tacchini-Cottier, F.

In: Infection and Immunity, Vol. 76, No. 2, 02.2008, p. 532-541.

Research output: Contribution to journalArticle

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