Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra.

Dominique A Daels-Rakotoarison, Veronique Seidel, Bernard Gressier, Claude Brunet, Francois Tillequin, Francois Bailleul, Michel Luyckx, Thierry Dine, Micheline Cazin, Jean-Claude Cazin

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Ballota nigra is a European plant known for its neurosedative properties. In this study, the ability of five phenylpropanoids (verbascoside, forsythoside B, arenarioside, ballotetroside, and caffeoyl malic acid) isolated from a hydroalcoholic extract, to bind to benzodiazepine, dopaminergic, and morphinic receptors was investigated. To carry out these studies, affinity tests with rat striata, entire brains and receptor rich preparations were employed. In addition, the phenolic aspect of these five phenylpropanoid esters led to investigate antioxidant activities using cell-free experiments and cellular experiments including isolated polymorphonuclear neutrophils (PMN). Effects of phenylpropanoid esters against reactive oxygen species as superoxide anion, peroxide hydrogen, hypochlorous acid and hydroxyl radical were tested. These molecules are liberated by PMN during inflammatory disorders, so that reproduction of this process in vitro stimulating PMN by chemical stimulants was undertaken. Results show that four of the five compounds are able to bind to the studied receptors. Inhibitory concentrations at 50% were determined and vary from 0.4 to 4.7 mg/ml. This may be in relation with the Ballota nigra known neurosedative activities. Results concerning antioxidant investigations evidence an ability to scavenge reactive oxygen species. Inhibitory concentrations at 50% obtained are comparable to those of known antioxidant drugs (mesna or N-acetyl cysteine). Moreover, the use of different stimuli having various pathways of action on PMN oxidative metabolism permits to establish that each phenylpropanoid ester has its own particular way of action by using proteine kinase C or phospholipase C pathways.
LanguageEnglish
Pages16-23
Number of pages8
JournalArzneimittel Forschung
Volume50
Issue number1
DOIs
Publication statusPublished - 31 Jan 2000

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Ballota
Neutrophils
Antioxidants
Esters
Inhibitory Concentration 50
Reactive Oxygen Species
Mesna
Hypochlorous Acid
Type C Phospholipases
Benzodiazepines
Superoxides
Hydroxyl Radical
Hydrogen Peroxide
Reproduction
Cysteine
Phosphotransferases
Brain
Pharmaceutical Preparations

Keywords

  • phenylpropanoids
  • reactive oxygen species
  • neurosedative activity
  • antioxidant activity

Cite this

Daels-Rakotoarison, D. A., Seidel, V., Gressier, B., Brunet, C., Tillequin, F., Bailleul, F., ... Cazin, J-C. (2000). Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra. Arzneimittel Forschung, 50(1), 16-23. https://doi.org/10.1055/s-0031-1300158
Daels-Rakotoarison, Dominique A ; Seidel, Veronique ; Gressier, Bernard ; Brunet, Claude ; Tillequin, Francois ; Bailleul, Francois ; Luyckx, Michel ; Dine, Thierry ; Cazin, Micheline ; Cazin, Jean-Claude. / Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra. In: Arzneimittel Forschung. 2000 ; Vol. 50, No. 1. pp. 16-23.
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Daels-Rakotoarison, DA, Seidel, V, Gressier, B, Brunet, C, Tillequin, F, Bailleul, F, Luyckx, M, Dine, T, Cazin, M & Cazin, J-C 2000, 'Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra.' Arzneimittel Forschung, vol. 50, no. 1, pp. 16-23. https://doi.org/10.1055/s-0031-1300158

Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra. / Daels-Rakotoarison, Dominique A; Seidel, Veronique; Gressier, Bernard; Brunet, Claude; Tillequin, Francois; Bailleul, Francois; Luyckx, Michel; Dine, Thierry ; Cazin, Micheline ; Cazin, Jean-Claude.

In: Arzneimittel Forschung, Vol. 50, No. 1, 31.01.2000, p. 16-23.

Research output: Contribution to journalArticle

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T1 - Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra.

AU - Daels-Rakotoarison, Dominique A

AU - Seidel, Veronique

AU - Gressier, Bernard

AU - Brunet, Claude

AU - Tillequin, Francois

AU - Bailleul, Francois

AU - Luyckx, Michel

AU - Dine, Thierry

AU - Cazin, Micheline

AU - Cazin, Jean-Claude

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N2 - Ballota nigra is a European plant known for its neurosedative properties. In this study, the ability of five phenylpropanoids (verbascoside, forsythoside B, arenarioside, ballotetroside, and caffeoyl malic acid) isolated from a hydroalcoholic extract, to bind to benzodiazepine, dopaminergic, and morphinic receptors was investigated. To carry out these studies, affinity tests with rat striata, entire brains and receptor rich preparations were employed. In addition, the phenolic aspect of these five phenylpropanoid esters led to investigate antioxidant activities using cell-free experiments and cellular experiments including isolated polymorphonuclear neutrophils (PMN). Effects of phenylpropanoid esters against reactive oxygen species as superoxide anion, peroxide hydrogen, hypochlorous acid and hydroxyl radical were tested. These molecules are liberated by PMN during inflammatory disorders, so that reproduction of this process in vitro stimulating PMN by chemical stimulants was undertaken. Results show that four of the five compounds are able to bind to the studied receptors. Inhibitory concentrations at 50% were determined and vary from 0.4 to 4.7 mg/ml. This may be in relation with the Ballota nigra known neurosedative activities. Results concerning antioxidant investigations evidence an ability to scavenge reactive oxygen species. Inhibitory concentrations at 50% obtained are comparable to those of known antioxidant drugs (mesna or N-acetyl cysteine). Moreover, the use of different stimuli having various pathways of action on PMN oxidative metabolism permits to establish that each phenylpropanoid ester has its own particular way of action by using proteine kinase C or phospholipase C pathways.

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KW - antioxidant activity

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Daels-Rakotoarison DA, Seidel V, Gressier B, Brunet C, Tillequin F, Bailleul F et al. Neurosedative and antioxidant activities of phenylpropanoids from Ballota nigra. Arzneimittel Forschung. 2000 Jan 31;50(1):16-23. https://doi.org/10.1055/s-0031-1300158