Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration

M. Pilar Calatayud, Christina Riggio, Vittoria Raffa, Beatrice Sanz, Teobaldo Torres, M. Ricardo Ibarra, Clare Hoskins, Alfred Cuschieri, Lijun Wang, Josephine Pinkernelle, Gerburg Khielhoff, Gerardo Goya

Research output: Contribution to journalArticle

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Abstract

We report a one-step synthesis protocol for obtaining polymer-coated magnetic nanoparticles (MNPs) engineered for uploading neural cells. Polyethyleneimine-coated Fe3O4 nanoparticles (PEI-MNPs) with sizes of 25 ± 5 nm were prepared by oxidation of Fe(OH)2 by nitrate in basic aqueous media and adding PEI in situ during synthesis. The obtained PEI-MNP cores displayed a neat octahedral morphology and high crystallinity. The resulting nanoparticles were coated with a thin polymer layer of about 0.7–0.9 nm, and displayed a saturation magnetization value MS = 58 A m2 kg−1 at 250 K (64 A m2 kg−1 for T = 10 K). Cell uptake experiments on a neuroblastoma-derived SH-SY5Y cell line were undertaken over a wide time and MNP concentration range. The results showed a small decrease in cell viability for 24 h incubation (down to 70% viability for 100 μg ml−1), increasing the toxic effects with incubation time (30% cell survival at 100 μg ml−1 for 7 days of incubation). On the other hand, primary neuronal cells displayed higher sensitivity to PEI-MNPs, with a cell viability reduction of 44% of the control cells after 3 days of incubation with 50 μg ml−1. The amount of PEI-MNPs uploaded by SH-SY5Y cells was found to have a linear dependence on concentration. The intracellular distribution of the PEI-MNPs analyzed at the single-cell level by the dual-beam (FIB/SEM) technique revealed the coexistence of both fully incorporated PEI-MNPs and partially internalized PEI-MNP-clusters crossing the cell membrane. The resulting MNP-cluster distributions open the possibility of using these PEI-MNPs for magnetically driven axonal re-growth in neural cells.
LanguageEnglish
Pages3607–3616
Number of pages10
JournalJournal of Materials Chemistry B
Volume1
Issue number29
DOIs
Publication statusPublished - 20 May 2013

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Polyetherimides
Nerve Regeneration
Nanoparticles
Polyethyleneimine
Cells
Cell Survival
Polymers
Poisons
Saturation magnetization
Cell membranes
Neuroblastoma
Nitrates

Keywords

  • neuronal cells
  • polymer-coated nanoparticles
  • magnetic nanoparticles
  • nerve regeneration

Cite this

Calatayud, M. P., Riggio, C., Raffa, V., Sanz, B., Torres, T., Ibarra, M. R., ... Goya, G. (2013). Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration. Journal of Materials Chemistry B, 1(29), 3607–3616. https://doi.org/10.1039/C3TB20336K
Calatayud, M. Pilar ; Riggio, Christina ; Raffa, Vittoria ; Sanz, Beatrice ; Torres, Teobaldo ; Ibarra, M. Ricardo ; Hoskins, Clare ; Cuschieri, Alfred ; Wang, Lijun ; Pinkernelle, Josephine ; Khielhoff, Gerburg ; Goya, Gerardo. / Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration. In: Journal of Materials Chemistry B. 2013 ; Vol. 1, No. 29. pp. 3607–3616.
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abstract = "We report a one-step synthesis protocol for obtaining polymer-coated magnetic nanoparticles (MNPs) engineered for uploading neural cells. Polyethyleneimine-coated Fe3O4 nanoparticles (PEI-MNPs) with sizes of 25 ± 5 nm were prepared by oxidation of Fe(OH)2 by nitrate in basic aqueous media and adding PEI in situ during synthesis. The obtained PEI-MNP cores displayed a neat octahedral morphology and high crystallinity. The resulting nanoparticles were coated with a thin polymer layer of about 0.7–0.9 nm, and displayed a saturation magnetization value MS = 58 A m2 kg−1 at 250 K (64 A m2 kg−1 for T = 10 K). Cell uptake experiments on a neuroblastoma-derived SH-SY5Y cell line were undertaken over a wide time and MNP concentration range. The results showed a small decrease in cell viability for 24 h incubation (down to 70{\%} viability for 100 μg ml−1), increasing the toxic effects with incubation time (30{\%} cell survival at 100 μg ml−1 for 7 days of incubation). On the other hand, primary neuronal cells displayed higher sensitivity to PEI-MNPs, with a cell viability reduction of 44{\%} of the control cells after 3 days of incubation with 50 μg ml−1. The amount of PEI-MNPs uploaded by SH-SY5Y cells was found to have a linear dependence on concentration. The intracellular distribution of the PEI-MNPs analyzed at the single-cell level by the dual-beam (FIB/SEM) technique revealed the coexistence of both fully incorporated PEI-MNPs and partially internalized PEI-MNP-clusters crossing the cell membrane. The resulting MNP-cluster distributions open the possibility of using these PEI-MNPs for magnetically driven axonal re-growth in neural cells.",
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Calatayud, MP, Riggio, C, Raffa, V, Sanz, B, Torres, T, Ibarra, MR, Hoskins, C, Cuschieri, A, Wang, L, Pinkernelle, J, Khielhoff, G & Goya, G 2013, 'Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration' Journal of Materials Chemistry B, vol. 1, no. 29, pp. 3607–3616. https://doi.org/10.1039/C3TB20336K

Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration. / Calatayud, M. Pilar; Riggio, Christina; Raffa, Vittoria; Sanz, Beatrice; Torres, Teobaldo; Ibarra, M. Ricardo; Hoskins, Clare; Cuschieri, Alfred; Wang, Lijun; Pinkernelle, Josephine; Khielhoff, Gerburg; Goya, Gerardo.

In: Journal of Materials Chemistry B, Vol. 1, No. 29, 20.05.2013, p. 3607–3616.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuronal cells loaded with PEI-coated Fe3O4 nanoparticles for magnetically guided nerve regeneration

AU - Calatayud, M. Pilar

AU - Riggio, Christina

AU - Raffa, Vittoria

AU - Sanz, Beatrice

AU - Torres, Teobaldo

AU - Ibarra, M. Ricardo

AU - Hoskins, Clare

AU - Cuschieri, Alfred

AU - Wang, Lijun

AU - Pinkernelle, Josephine

AU - Khielhoff, Gerburg

AU - Goya, Gerardo

PY - 2013/5/20

Y1 - 2013/5/20

N2 - We report a one-step synthesis protocol for obtaining polymer-coated magnetic nanoparticles (MNPs) engineered for uploading neural cells. Polyethyleneimine-coated Fe3O4 nanoparticles (PEI-MNPs) with sizes of 25 ± 5 nm were prepared by oxidation of Fe(OH)2 by nitrate in basic aqueous media and adding PEI in situ during synthesis. The obtained PEI-MNP cores displayed a neat octahedral morphology and high crystallinity. The resulting nanoparticles were coated with a thin polymer layer of about 0.7–0.9 nm, and displayed a saturation magnetization value MS = 58 A m2 kg−1 at 250 K (64 A m2 kg−1 for T = 10 K). Cell uptake experiments on a neuroblastoma-derived SH-SY5Y cell line were undertaken over a wide time and MNP concentration range. The results showed a small decrease in cell viability for 24 h incubation (down to 70% viability for 100 μg ml−1), increasing the toxic effects with incubation time (30% cell survival at 100 μg ml−1 for 7 days of incubation). On the other hand, primary neuronal cells displayed higher sensitivity to PEI-MNPs, with a cell viability reduction of 44% of the control cells after 3 days of incubation with 50 μg ml−1. The amount of PEI-MNPs uploaded by SH-SY5Y cells was found to have a linear dependence on concentration. The intracellular distribution of the PEI-MNPs analyzed at the single-cell level by the dual-beam (FIB/SEM) technique revealed the coexistence of both fully incorporated PEI-MNPs and partially internalized PEI-MNP-clusters crossing the cell membrane. The resulting MNP-cluster distributions open the possibility of using these PEI-MNPs for magnetically driven axonal re-growth in neural cells.

AB - We report a one-step synthesis protocol for obtaining polymer-coated magnetic nanoparticles (MNPs) engineered for uploading neural cells. Polyethyleneimine-coated Fe3O4 nanoparticles (PEI-MNPs) with sizes of 25 ± 5 nm were prepared by oxidation of Fe(OH)2 by nitrate in basic aqueous media and adding PEI in situ during synthesis. The obtained PEI-MNP cores displayed a neat octahedral morphology and high crystallinity. The resulting nanoparticles were coated with a thin polymer layer of about 0.7–0.9 nm, and displayed a saturation magnetization value MS = 58 A m2 kg−1 at 250 K (64 A m2 kg−1 for T = 10 K). Cell uptake experiments on a neuroblastoma-derived SH-SY5Y cell line were undertaken over a wide time and MNP concentration range. The results showed a small decrease in cell viability for 24 h incubation (down to 70% viability for 100 μg ml−1), increasing the toxic effects with incubation time (30% cell survival at 100 μg ml−1 for 7 days of incubation). On the other hand, primary neuronal cells displayed higher sensitivity to PEI-MNPs, with a cell viability reduction of 44% of the control cells after 3 days of incubation with 50 μg ml−1. The amount of PEI-MNPs uploaded by SH-SY5Y cells was found to have a linear dependence on concentration. The intracellular distribution of the PEI-MNPs analyzed at the single-cell level by the dual-beam (FIB/SEM) technique revealed the coexistence of both fully incorporated PEI-MNPs and partially internalized PEI-MNP-clusters crossing the cell membrane. The resulting MNP-cluster distributions open the possibility of using these PEI-MNPs for magnetically driven axonal re-growth in neural cells.

KW - neuronal cells

KW - polymer-coated nanoparticles

KW - magnetic nanoparticles

KW - nerve regeneration

U2 - 10.1039/C3TB20336K

DO - 10.1039/C3TB20336K

M3 - Article

VL - 1

SP - 3607

EP - 3616

JO - Journal of Materials Chemistry B

T2 - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-750X

IS - 29

ER -