Neuromuscular effects of fractions isolated from the venom of the Thailand cobra, Naja naja siamensis

P. Teerapong, A. L. Harvey, T. Barkas

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Four fractions (α, β, γ and δ) were isolated from the venom of the Thailand cobra, Naja naja siamensis by ion-exchange chromatography. Each fraction was tested for activity on the isolated chick biventer cervicis nerve-muscle preparation. Fractions α, β and γ inhibited neuromuscular transmission by blocking acetylcholine receptors, but had no direct action on muscle contractility. Fraction α produced neuromuscular blockade in about 30 min when added at a concentration of 1 μg/ml, and was about 100 times more potent than fractions β and γ. The blockade of indirect stimulation was apparently irreversible, although the responses to acetylcholine and carbachol recovered to about 50% after 4 hr. Fraction δ produced contracture of the muscle followed by blockade of responses to both direct and indirect stimulation. The effects of fraction δ could be inhibited by raising the calcium concentration of the bathing solution; on return to normal calcium concentration the effects of fraction δ also returned. It is concluded that fractions α, β and γ are postjunctional "neurotoxins" and that fraction δ contains a "cardiotoxin" which may act by displacing calcium from membranes.

LanguageEnglish
Pages551-560
Number of pages10
JournalToxicon
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Jan 1978

Fingerprint

Neuromuscular Agents
Cobra Venoms
Elapidae
Venoms
Thailand
Muscle
Calcium
Cardiotoxins
Muscles
Neuromuscular Blockade
Neuromuscular Junction
Ion Exchange Chromatography
Neurotoxins
Carbachol
Contracture
Cholinergic Receptors
Chromatography
Acetylcholine
Ion exchange
Membranes

Keywords

  • acetylcholine
  • alpha bungarotoxin
  • carbachol
  • cholinergic receptor
  • neostigmine
  • snake venom
  • tubocurarine chloride
  • animal experiment
  • chicken
  • muscle
  • neuromuscular synapse
  • neuromuscular transmission
  • peripheral nervous system
  • reptile

Cite this

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title = "Neuromuscular effects of fractions isolated from the venom of the Thailand cobra, Naja naja siamensis",
abstract = "Four fractions (α, β, γ and δ) were isolated from the venom of the Thailand cobra, Naja naja siamensis by ion-exchange chromatography. Each fraction was tested for activity on the isolated chick biventer cervicis nerve-muscle preparation. Fractions α, β and γ inhibited neuromuscular transmission by blocking acetylcholine receptors, but had no direct action on muscle contractility. Fraction α produced neuromuscular blockade in about 30 min when added at a concentration of 1 μg/ml, and was about 100 times more potent than fractions β and γ. The blockade of indirect stimulation was apparently irreversible, although the responses to acetylcholine and carbachol recovered to about 50{\%} after 4 hr. Fraction δ produced contracture of the muscle followed by blockade of responses to both direct and indirect stimulation. The effects of fraction δ could be inhibited by raising the calcium concentration of the bathing solution; on return to normal calcium concentration the effects of fraction δ also returned. It is concluded that fractions α, β and γ are postjunctional {"}neurotoxins{"} and that fraction δ contains a {"}cardiotoxin{"} which may act by displacing calcium from membranes.",
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Neuromuscular effects of fractions isolated from the venom of the Thailand cobra, Naja naja siamensis. / Teerapong, P.; Harvey, A. L.; Barkas, T.

In: Toxicon, Vol. 16, No. 6, 01.01.1978, p. 551-560.

Research output: Contribution to journalArticle

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