Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis

Chesney E. Michels, Hannah E. Scales, Karin A. Saunders, S. McGowan, Frank Brombracher, James Alexander, Catherine E. Lawrence

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.
Original languageEnglish
Pages (from-to)e385-e394
Number of pages10
JournalImmunology
Volume128
Issue number1pt2
Early online date16 Dec 2008
DOIs
Publication statusPublished - Sep 2009

Keywords

  • cell-specific interleukin-4 receptor α transgenic mice
  • gastrointestinal nematodes
  • mastocytosis
  • T helper immune response
  • trichinella spiralis

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