Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis

Chesney E. Michels, Hannah E. Scales, Karin A. Saunders, S. McGowan, Frank Brombracher, James Alexander, Catherine E. Lawrence

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.
LanguageEnglish
Pagese385-e394
Number of pages10
JournalImmunology
Volume128
Issue number1pt2
Early online date16 Dec 2008
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Interleukin-4 Receptor alpha Subunit
Trichinella spiralis
Immunity
Neutrophils
Macrophages
T-Lymphocytes
Mastocytosis
Interleukin-4
Parasites
Bone Marrow
Interleukin-13
Helminths
Infection Control
Immunoglobulin E
Antibody Formation
Immunoglobulins

Keywords

  • cell-specific interleukin-4 receptor α transgenic mice
  • gastrointestinal nematodes
  • mastocytosis
  • T helper immune response
  • trichinella spiralis

Cite this

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title = "Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis",
abstract = "The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.",
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Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis. / Michels, Chesney E.; Scales, Hannah E.; Saunders, Karin A.; McGowan, S.; Brombracher, Frank; Alexander, James; Lawrence, Catherine E.

In: Immunology, Vol. 128, No. 1pt2, 09.2009, p. e385-e394.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neither interleukin-4 receptor α expression on CD4+ T cells, or macrophages and neutrophils is required for protective immunity to Trichinella spiralis

AU - Michels, Chesney E.

AU - Scales, Hannah E.

AU - Saunders, Karin A.

AU - McGowan, S.

AU - Brombracher, Frank

AU - Alexander, James

AU - Lawrence, Catherine E.

PY - 2009/9

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N2 - The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.

AB - The T helper type 2 (Th2) mediated expulsion of the gastrointestinal nematode Trichinella spiralis requires interleukin-4 receptor α (IL-4Rα) expression on both bone-marrow-derived and non-bone-marrow-derived cells. To more definitively investigate the role of IL-4/IL-13 responsiveness in the development of protective immunity to T. spiralis, cell-specific IL-4Rα signalling on CD4+ T cells (Lckcre IL-4Rα−/flox) and macrophages/neutrophils (LysMcre IL-4Rα−/flox) was analysed on the BALB/c background. Infection of wild-type and control IL-4Rα−/flox mice induced a Th2-type immune response with elevated IL-4 cytokine production, parasite-specific immunoglobulin G1 (IgG1), total IgE, intestinal mastocytosis and enteropathy. In contrast, global IL-4Rα-deficient BALB/c mice showed reduced worm expulsion, antibody production, intestinal mastocytosis and gut pathology. BALB/c mice generated with cell-specific deletion of IL-4Rα on CD4+ T lymphocytes or macrophages/neutrophils, controlled gastrointestinal helminth infection by eliciting a protective immune response comparable to that observed with wild-type and IL-4Rα−/flox controls. Together, this shows that the development of host protective Th2 responses accompanied by parasite loss is independent of IL-4Rα expression on CD4+ T cells and macrophages/neutrophils.

KW - cell-specific interleukin-4 receptor α transgenic mice

KW - gastrointestinal nematodes

KW - mastocytosis

KW - T helper immune response

KW - trichinella spiralis

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DO - 10.1111/j.1365-2567.2008.02987.x

M3 - Article

VL - 128

SP - e385-e394

JO - Immunology

T2 - Immunology

JF - Immunology

SN - 0019-2805

IS - 1pt2

ER -