Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture

B. McNeil, B. Finn, L.M. Harvey

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The use of at-line NIRS to monitor a high cell density fed-batch baker's yeast bioprocess was investigated. Quantification of the key analytes (biomass, ethanol and glucose) and the product quality indicator (percentage protein content) was studied. Biomass was quantitatively modelled using whole matrix samples (as was percentage protein content). The dominance of the whole matrix spectrum by biomass, and its associated light scattering effects, were overcome by use of filtrate samples and adapted (semi-synthetic) filtrate samples, which allowed successful ethanol and glucose modelling, respectively. Calibrations were rigorously challenged via external validation with large sample sets relative to the calibration sample size, ensuring model robustness and potential practical utility. The standard errors of calibration for biomass, glucose, ethanol and total intracellular protein were (g/l) 1.79, 0.19, 0.79 and 0.91, respectively, comparable to those of the primary assays. The calibration strategies necessary to generate quantitative models for this range of analytes in such a complex high cell density bioprocess fluid are discussed.
LanguageEnglish
Pages507-517
Number of pages10
JournalYeast
Volume23
Issue number7
DOIs
Publication statusPublished - 2006

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Batch Cell Culture Techniques
Yeast
Biomass
Calibration
Glucose
Saccharomyces cerevisiae
Ethanol
Cell Count
Infrared radiation
Proteins
Monitoring
Light scattering
Sample Size
Assays
Light
Fluids

Keywords

  • near-infrared spectroscopy
  • saccharomyces cerevisiae
  • bioprocess monitoring
  • high cell density
  • fed-batch bioprocess
  • pharmacology
  • biomedical sciences

Cite this

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Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture. / McNeil, B.; Finn, B.; Harvey, L.M.

In: Yeast, Vol. 23, No. 7, 2006, p. 507-517.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Near infrared spectroscopic monitoring biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch culture

AU - McNeil, B.

AU - Finn, B.

AU - Harvey, L.M.

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AB - The use of at-line NIRS to monitor a high cell density fed-batch baker's yeast bioprocess was investigated. Quantification of the key analytes (biomass, ethanol and glucose) and the product quality indicator (percentage protein content) was studied. Biomass was quantitatively modelled using whole matrix samples (as was percentage protein content). The dominance of the whole matrix spectrum by biomass, and its associated light scattering effects, were overcome by use of filtrate samples and adapted (semi-synthetic) filtrate samples, which allowed successful ethanol and glucose modelling, respectively. Calibrations were rigorously challenged via external validation with large sample sets relative to the calibration sample size, ensuring model robustness and potential practical utility. The standard errors of calibration for biomass, glucose, ethanol and total intracellular protein were (g/l) 1.79, 0.19, 0.79 and 0.91, respectively, comparable to those of the primary assays. The calibration strategies necessary to generate quantitative models for this range of analytes in such a complex high cell density bioprocess fluid are discussed.

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KW - saccharomyces cerevisiae

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KW - fed-batch bioprocess

KW - pharmacology

KW - biomedical sciences

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