Near-infrared fluorescence lifetime assay for serum glucose based on allophycocyanin-labeled concanavalin A

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the nearinfrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran- MG and leads to reduction in FRET, assessed by time-domain fluorescence lifetime measurements using time-correlated single-photon counting. The assay is operative in the glucose concentration range 2.5-30 mM, and therefore suitable for use in monitoring diabetes control. Albumin and serum inhibit FRET but the interference can be prevented by removal of high molecular weight substances by membrane filters. APC shows promise for incorporation in an implanted glucose sensor which can be interrogated from outside the body.
LanguageEnglish
Pages216-221
Number of pages5
JournalAnalytical Biochemistry
Volume292
Issue number2
DOIs
Publication statusPublished - 15 May 2001

Fingerprint

Concanavalin A
Assays
Fluorescence
Dextrans
Infrared radiation
Glucose
Serum
Energy Transfer
Photons
Serum Albumin
Energy transfer
Membranes
Weights and Measures
Monitoring
Sensors
allophycocyanin
Proteins
malachite green

Keywords

  • fluorescence
  • lifetime assay
  • serum glucose
  • nanoscience
  • allophycocyanin

Cite this

@article{6154b9f42a9d46e08caf034fe4752bd7,
title = "Near-infrared fluorescence lifetime assay for serum glucose based on allophycocyanin-labeled concanavalin A",
abstract = "We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the nearinfrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran- MG and leads to reduction in FRET, assessed by time-domain fluorescence lifetime measurements using time-correlated single-photon counting. The assay is operative in the glucose concentration range 2.5-30 mM, and therefore suitable for use in monitoring diabetes control. Albumin and serum inhibit FRET but the interference can be prevented by removal of high molecular weight substances by membrane filters. APC shows promise for incorporation in an implanted glucose sensor which can be interrogated from outside the body.",
keywords = "fluorescence, lifetime assay, serum glucose, nanoscience, allophycocyanin",
author = "McCartney, {Lydia J.} and Pickup, {John C.} and Rolinski, {Olaf J.} and Birch, {David J.S.}",
year = "2001",
month = "5",
day = "15",
doi = "10.1006/abio.2001.5060",
language = "English",
volume = "292",
pages = "216--221",
journal = "Analytical Biochemistry",
issn = "0003-2697",
number = "2",

}

TY - JOUR

T1 - Near-infrared fluorescence lifetime assay for serum glucose based on allophycocyanin-labeled concanavalin A

AU - McCartney, Lydia J.

AU - Pickup, John C.

AU - Rolinski, Olaf J.

AU - Birch, David J.S.

PY - 2001/5/15

Y1 - 2001/5/15

N2 - We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the nearinfrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran- MG and leads to reduction in FRET, assessed by time-domain fluorescence lifetime measurements using time-correlated single-photon counting. The assay is operative in the glucose concentration range 2.5-30 mM, and therefore suitable for use in monitoring diabetes control. Albumin and serum inhibit FRET but the interference can be prevented by removal of high molecular weight substances by membrane filters. APC shows promise for incorporation in an implanted glucose sensor which can be interrogated from outside the body.

AB - We describe an assay scheme for glucose based on fluorescence resonance energy transfer (FRET) between concanavalin A (con A), labeled with the nearinfrared fluorescent protein allophycocyanin (APC) as donor, and dextran labeled with malachite green (MG) as acceptor. Glucose competitively displaces dextran- MG and leads to reduction in FRET, assessed by time-domain fluorescence lifetime measurements using time-correlated single-photon counting. The assay is operative in the glucose concentration range 2.5-30 mM, and therefore suitable for use in monitoring diabetes control. Albumin and serum inhibit FRET but the interference can be prevented by removal of high molecular weight substances by membrane filters. APC shows promise for incorporation in an implanted glucose sensor which can be interrogated from outside the body.

KW - fluorescence

KW - lifetime assay

KW - serum glucose

KW - nanoscience

KW - allophycocyanin

UR - http://www.idealibrary.com

U2 - 10.1006/abio.2001.5060

DO - 10.1006/abio.2001.5060

M3 - Article

VL - 292

SP - 216

EP - 221

JO - Analytical Biochemistry

T2 - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -