Natural products modulate Shigella–host-cell interaction

Dan Xu, Amir Saeed, Yili Wang, Veronique Seidel, Gunnar Sandstrome, Jun Yu

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1 Citation (Scopus)

Abstract

This study focused on identifying possible new options derived from natural sources for the treatment of bacterial infections. Several natural products were investigated for their potential in modulating Shigella-host-cell interactions. The proliferation of Shigella sonnei was effectively inhibited inside HEp-2 cells in the presence of 4-methoxycinnamic acid and propolin D. Propolin D also significantly reduced the apoptosis of infected macrophage-like U937 cells and moderately reduced the secretion of interleukin (IL)-1β and IL-18, which probably resulted from the inhibition of invasion plasmid antigen B secretion by this compound. Further characterization showed that propolin D did not prevent escape of Shigella from phagocytic vacuoles, as evidenced by actin-based motility and by the fact that addition of chloroquine did not further reduce the number of intracellular c.f.u. The role of propolin D in modulating autophagy could not be established under the experimental conditions used. As these compounds had no direct anti-Shigella activity in vitro, it was concluded that these compounds modulated Shigella-host-cell interactions by targeting yet-to-be defined mechanisms that provide benefits to host cells.

Original languageEnglish
Pages (from-to)1626-1632
Number of pages7
JournalJournal of Medical Microbiology
Volume60
Issue number11
Early online date30 Jun 2011
DOIs
Publication statusPublished - Nov 2011

Keywords

  • natual products
  • shigella sonnei
  • host interation

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