Natural and synthetic polyesters for musculoskeletal tissue repair: experimental in vitro and in vivo evaluations

G. Giavaresi, M. Tschon, J.H. Daly, J.J. Liggat, M. Fini, P. Torricelli, R. Giardino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two natural Biopol(TM) polyesters, containing 8% (D400G) and 12% (D600G) of hydroxyvalerate component, and a synthetic polyester based on 1,4 cyclohexanediol [Poly(cyclohexyl-sebacate) - PCS] were studied to investigate their in vitro and in vivo behavior for application in musculoskeletal tissue repair. The polyesters were placed in direct contact with L929 fibroblasts and cell proliferation (WST-1), cytotoxic effect (LDH), synthetic activity (total proteins) and cytokine production (IL-1beta, IL-6, TNFalpha) were assessed after an incubation period of 72 hours and 7 days. Then, 12 Sprague-Dawley rats underwent dorsal subcutaneous implants of tested polyesters under general anesthesia. After 1 and 4 weeks from surgery, the animals were pharmacologically euthanized and the implants retrieved with surrounding tissue for histologic and histomorphometric investigations. In vitro results showed that D600G behaved a little worse in comparison to other tested polyesters in terms of cell proliferation and TNFalpha at 7 days. PCS presented the lowest total protein value at 7 days. In vivo results indicated that PCS implants produced a higher (p < 0.01) extent of inflammatoty tissue in comparison to D600G at 1 week and to D40OG at 4 weeks, and the lowest vascular densities at both experimental times. D40OG seems to be the most suitable material for biomedical application when tested in fibroblast cultures and in the subcutaneous tissue of rats.
LanguageEnglish
Pages796-805
Number of pages9
JournalInternational Journal of Artificial Organs
Volume27
Issue number9
Publication statusPublished - Sep 2004

Fingerprint

Polyesters
Repair
Tissue
Cell proliferation
Fibroblasts
Rats
Tumor Necrosis Factor-alpha
Cell Proliferation
Proteins
Subcutaneous Tissue
Cell culture
General Anesthesia
Surgery
Blood Vessels
Sprague Dawley Rats
Interleukin-6
Animals
In Vitro Techniques
Cytokines

Keywords

  • polyesters
  • murine fibroblasts
  • animal model
  • biocompatibility
  • implant

Cite this

Giavaresi, G. ; Tschon, M. ; Daly, J.H. ; Liggat, J.J. ; Fini, M. ; Torricelli, P. ; Giardino, R. / Natural and synthetic polyesters for musculoskeletal tissue repair: experimental in vitro and in vivo evaluations. In: International Journal of Artificial Organs. 2004 ; Vol. 27, No. 9. pp. 796-805.
@article{94e8e6d0148048b5b4f81e6f9839e53d,
title = "Natural and synthetic polyesters for musculoskeletal tissue repair: experimental in vitro and in vivo evaluations",
abstract = "Two natural Biopol(TM) polyesters, containing 8{\%} (D400G) and 12{\%} (D600G) of hydroxyvalerate component, and a synthetic polyester based on 1,4 cyclohexanediol [Poly(cyclohexyl-sebacate) - PCS] were studied to investigate their in vitro and in vivo behavior for application in musculoskeletal tissue repair. The polyesters were placed in direct contact with L929 fibroblasts and cell proliferation (WST-1), cytotoxic effect (LDH), synthetic activity (total proteins) and cytokine production (IL-1beta, IL-6, TNFalpha) were assessed after an incubation period of 72 hours and 7 days. Then, 12 Sprague-Dawley rats underwent dorsal subcutaneous implants of tested polyesters under general anesthesia. After 1 and 4 weeks from surgery, the animals were pharmacologically euthanized and the implants retrieved with surrounding tissue for histologic and histomorphometric investigations. In vitro results showed that D600G behaved a little worse in comparison to other tested polyesters in terms of cell proliferation and TNFalpha at 7 days. PCS presented the lowest total protein value at 7 days. In vivo results indicated that PCS implants produced a higher (p < 0.01) extent of inflammatoty tissue in comparison to D600G at 1 week and to D40OG at 4 weeks, and the lowest vascular densities at both experimental times. D40OG seems to be the most suitable material for biomedical application when tested in fibroblast cultures and in the subcutaneous tissue of rats.",
keywords = "polyesters, murine fibroblasts, animal model, biocompatibility, implant",
author = "G. Giavaresi and M. Tschon and J.H. Daly and J.J. Liggat and M. Fini and P. Torricelli and R. Giardino",
year = "2004",
month = "9",
language = "English",
volume = "27",
pages = "796--805",
journal = "International Journal of Artificial Organs",
issn = "0391-3988",
number = "9",

}

Natural and synthetic polyesters for musculoskeletal tissue repair: experimental in vitro and in vivo evaluations. / Giavaresi, G.; Tschon, M.; Daly, J.H.; Liggat, J.J.; Fini, M.; Torricelli, P.; Giardino, R.

In: International Journal of Artificial Organs, Vol. 27, No. 9, 09.2004, p. 796-805.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Natural and synthetic polyesters for musculoskeletal tissue repair: experimental in vitro and in vivo evaluations

AU - Giavaresi, G.

AU - Tschon, M.

AU - Daly, J.H.

AU - Liggat, J.J.

AU - Fini, M.

AU - Torricelli, P.

AU - Giardino, R.

PY - 2004/9

Y1 - 2004/9

N2 - Two natural Biopol(TM) polyesters, containing 8% (D400G) and 12% (D600G) of hydroxyvalerate component, and a synthetic polyester based on 1,4 cyclohexanediol [Poly(cyclohexyl-sebacate) - PCS] were studied to investigate their in vitro and in vivo behavior for application in musculoskeletal tissue repair. The polyesters were placed in direct contact with L929 fibroblasts and cell proliferation (WST-1), cytotoxic effect (LDH), synthetic activity (total proteins) and cytokine production (IL-1beta, IL-6, TNFalpha) were assessed after an incubation period of 72 hours and 7 days. Then, 12 Sprague-Dawley rats underwent dorsal subcutaneous implants of tested polyesters under general anesthesia. After 1 and 4 weeks from surgery, the animals were pharmacologically euthanized and the implants retrieved with surrounding tissue for histologic and histomorphometric investigations. In vitro results showed that D600G behaved a little worse in comparison to other tested polyesters in terms of cell proliferation and TNFalpha at 7 days. PCS presented the lowest total protein value at 7 days. In vivo results indicated that PCS implants produced a higher (p < 0.01) extent of inflammatoty tissue in comparison to D600G at 1 week and to D40OG at 4 weeks, and the lowest vascular densities at both experimental times. D40OG seems to be the most suitable material for biomedical application when tested in fibroblast cultures and in the subcutaneous tissue of rats.

AB - Two natural Biopol(TM) polyesters, containing 8% (D400G) and 12% (D600G) of hydroxyvalerate component, and a synthetic polyester based on 1,4 cyclohexanediol [Poly(cyclohexyl-sebacate) - PCS] were studied to investigate their in vitro and in vivo behavior for application in musculoskeletal tissue repair. The polyesters were placed in direct contact with L929 fibroblasts and cell proliferation (WST-1), cytotoxic effect (LDH), synthetic activity (total proteins) and cytokine production (IL-1beta, IL-6, TNFalpha) were assessed after an incubation period of 72 hours and 7 days. Then, 12 Sprague-Dawley rats underwent dorsal subcutaneous implants of tested polyesters under general anesthesia. After 1 and 4 weeks from surgery, the animals were pharmacologically euthanized and the implants retrieved with surrounding tissue for histologic and histomorphometric investigations. In vitro results showed that D600G behaved a little worse in comparison to other tested polyesters in terms of cell proliferation and TNFalpha at 7 days. PCS presented the lowest total protein value at 7 days. In vivo results indicated that PCS implants produced a higher (p < 0.01) extent of inflammatoty tissue in comparison to D600G at 1 week and to D40OG at 4 weeks, and the lowest vascular densities at both experimental times. D40OG seems to be the most suitable material for biomedical application when tested in fibroblast cultures and in the subcutaneous tissue of rats.

KW - polyesters

KW - murine fibroblasts

KW - animal model

KW - biocompatibility

KW - implant

M3 - Article

VL - 27

SP - 796

EP - 805

JO - International Journal of Artificial Organs

T2 - International Journal of Artificial Organs

JF - International Journal of Artificial Organs

SN - 0391-3988

IS - 9

ER -