Nanosensors for intracellular Raman studies

Patrick Thomson, Colin J. Campbell

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Raman spectroscopy is an increasingly exploited tool for the study of cell biology, and the focus of this chapter is surface-enhanced Raman spectroscopy (SERS). Using SERS, noble metal nanoparticles can be interrogated with wavelengths of light to which cells exhibit minimal autofluorescence and return useful information about their immediate chemical environment. Using established gold–thiol surface chemistry, gold nanoparticles can be functionalised with suitable reporter molecules used to make nanosensors, which can be chosen to be sensitive to intracellular variables such as pH, protein concentration/activity or redox potential. This chapter presents the general concept of SERS for use in the monitoring of intracellular variables, and we will describe the preparation, use and functions of the nanosensors.
LanguageEnglish
Title of host publication Lecture Notes in Nanoscale Science and Technology
Subtitle of host publicationNanoscale Sensors
Place of PublicationCham, Switzerland
PublisherSpringer
Pages35-54
Number of pages20
Volume19
ISBN (Print)978-3-319-02771-5
DOIs
Publication statusPublished - 12 Dec 2013

Fingerprint

Nanosensors
Raman spectroscopy
Gold
Cytology
Metal nanoparticles
Precious metals
Surface chemistry
Sulfhydryl Compounds
Nanoparticles
Wavelength
Molecules
Monitoring
Proteins

Keywords

  • nanosensors
  • Raman
  • SERS

Cite this

Thomson, P., & Campbell, C. J. (2013). Nanosensors for intracellular Raman studies. In Lecture Notes in Nanoscale Science and Technology: Nanoscale Sensors (Vol. 19, pp. 35-54). Cham, Switzerland: Springer. https://doi.org/10.1007/978-3-319-02772-2_2
Thomson, Patrick ; Campbell, Colin J. / Nanosensors for intracellular Raman studies. Lecture Notes in Nanoscale Science and Technology: Nanoscale Sensors. Vol. 19 Cham, Switzerland : Springer, 2013. pp. 35-54
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Thomson, P & Campbell, CJ 2013, Nanosensors for intracellular Raman studies. in Lecture Notes in Nanoscale Science and Technology: Nanoscale Sensors. vol. 19, Springer, Cham, Switzerland, pp. 35-54. https://doi.org/10.1007/978-3-319-02772-2_2

Nanosensors for intracellular Raman studies. / Thomson, Patrick; Campbell, Colin J.

Lecture Notes in Nanoscale Science and Technology: Nanoscale Sensors. Vol. 19 Cham, Switzerland : Springer, 2013. p. 35-54.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Thomson P, Campbell CJ. Nanosensors for intracellular Raman studies. In Lecture Notes in Nanoscale Science and Technology: Nanoscale Sensors. Vol. 19. Cham, Switzerland: Springer. 2013. p. 35-54 https://doi.org/10.1007/978-3-319-02772-2_2