Nanopropulsion by biocatalytic self-assembly

Joy Leckie, Alexander Hope, Meghan Hughes, Sisir Debnath, Scott Fleming, Alastair W. Wark, Rein V. Ulijn, Mark D. Haw

Research output: Contribution to journalArticle

13 Citations (Scopus)
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Abstract

A number of organisms and organelles are capable of self-propulsion at the micro- and nanoscales. Production of simple man-made mimics of biological transportation systems may prove relevant to achieving movement in artificial cells and nano/micronscale robotics that may be of biological and nanotechnological importance. We demonstrate the propulsion of particles based on catalytically controlled molecular self-assembly and fiber formation at the particle surface. Specifically, phosphatase enzymes (acting as the engine) are conjugated to a quantum dot (the vehicle), and are subsequently exposed to micellar aggregates (fuel) that upon biocatalytic dephosphorylation undergo fibrillar self-assembly, which in turn causes propulsion. The motion of individual enzyme/quantum dot conjugates is followed directly using fluorescence microscopy. While overall movement remains random, the enzymeconjugates exhibit significantly faster transport in the presence of the fiber forming system, compared to controls without fuel, a non-self-assembling substrate, or a substrate which assembles into spherical, rather than fibrous structures upon enzymatic dephosphorylation. When increasing the concentration of the fiber-forming fuel, the speed of the conjugates increases compared to non-self-assembling substrate, although directionality remains random.
Original languageEnglish
Pages (from-to)9580-9589
Number of pages10
JournalACS Nano
Volume8
Issue number9
Early online date27 Aug 2014
DOIs
Publication statusPublished - 23 Sep 2014

Fingerprint

propulsion
Self assembly
Propulsion
self assembly
assembling
Semiconductor quantum dots
fibers
Fibers
enzymes
fuel control
Substrates
Enzymes
Nanorobotics
quantum dots
organelles
phosphatases
Fluorescence microscopy
Phosphatases
robotics
organisms

Keywords

  • nanopropulsion
  • biocatalysis
  • self assembly
  • aromatic peptide amphiphiles
  • single particle tracking

Cite this

Leckie, J., Hope, A., Hughes, M., Debnath, S., Fleming, S., Wark, A. W., ... Haw, M. D. (2014). Nanopropulsion by biocatalytic self-assembly. ACS Nano, 8(9), 9580-9589. https://doi.org/10.1021/nn503875y
Leckie, Joy ; Hope, Alexander ; Hughes, Meghan ; Debnath, Sisir ; Fleming, Scott ; Wark, Alastair W. ; Ulijn, Rein V. ; Haw, Mark D. / Nanopropulsion by biocatalytic self-assembly. In: ACS Nano. 2014 ; Vol. 8, No. 9. pp. 9580-9589.
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Leckie, J, Hope, A, Hughes, M, Debnath, S, Fleming, S, Wark, AW, Ulijn, RV & Haw, MD 2014, 'Nanopropulsion by biocatalytic self-assembly', ACS Nano, vol. 8, no. 9, pp. 9580-9589. https://doi.org/10.1021/nn503875y

Nanopropulsion by biocatalytic self-assembly. / Leckie, Joy; Hope, Alexander; Hughes, Meghan; Debnath, Sisir; Fleming, Scott; Wark, Alastair W.; Ulijn, Rein V.; Haw, Mark D.

In: ACS Nano, Vol. 8, No. 9, 23.09.2014, p. 9580-9589.

Research output: Contribution to journalArticle

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AU - Hope, Alexander

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AU - Ulijn, Rein V.

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Leckie J, Hope A, Hughes M, Debnath S, Fleming S, Wark AW et al. Nanopropulsion by biocatalytic self-assembly. ACS Nano. 2014 Sep 23;8(9):9580-9589. https://doi.org/10.1021/nn503875y