Nanoparticle-mediated magnetic hyperthermia is an effective method for killing the human-infective protozoan parasite Leishmania mexicana in vitro

Sarah L. Berry, Karen Walker, Clare Hoskins, Neil D. Telling, Helen P. Price

Research output: Contribution to journalArticle

Abstract

Cutaneous leishmaniasis is a neglected tropical disease characterized by disfiguring skin lesions. Current chemotherapeutic options depend on toxic, expensive drugs that are both difficult to administer and becoming less effective due to increasing levels of resistance. In comparison, thermotherapy displays greater patient compliance and less adverse systemic effects, but there are still significant issues associated with this. The procedure is painful, requiring local anaesthetic, and is less effective against large lesions. Using nanoparticles to controllably generate heat in a localized manner may provide an alternative solution. Here we evaluate magnetic hyperthermia, using iron oxide magnetic nanoparticles, as a localized, heat-based method to kill the human-infective parasite in vitro. We assessed the effectiveness of this method against the differentiated, amastigote form of the parasite using three distinct viability assays: PrestoBlue, Live/Dead stain and a novel luciferase-based assay. Changes in amastigote morphology and ultrastructure were assessed by immunofluorescence, scanning and transmission electron microscopy. Our findings show that magnetic hyperthermia is an effective method to kill host-infective amastigotes, with morphological changes consistent with heat treatment. This method has the potential to be a step-change for research into new therapeutic options that moves away from the expensive chemotherapeutics currently dominating the research climate.
LanguageEnglish
Article number1059
Number of pages9
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 31 Jan 2019

Fingerprint

Leishmania mexicana
Nanoparticles
Parasites
Fever
Hot Temperature
Neglected Diseases
Scanning Transmission Electron Microscopy
Cutaneous Leishmaniasis
Induced Hyperthermia
Poisons
Patient Compliance
Local Anesthetics
Luciferases
Climate
Research
Fluorescent Antibody Technique
Coloring Agents
In Vitro Techniques
Skin
Therapeutics

Keywords

  • nanomedicine
  • nanoparticles
  • Cutaneous leishmaniasis
  • tropical diseases
  • magnetic hyperthermia

Cite this

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abstract = "Cutaneous leishmaniasis is a neglected tropical disease characterized by disfiguring skin lesions. Current chemotherapeutic options depend on toxic, expensive drugs that are both difficult to administer and becoming less effective due to increasing levels of resistance. In comparison, thermotherapy displays greater patient compliance and less adverse systemic effects, but there are still significant issues associated with this. The procedure is painful, requiring local anaesthetic, and is less effective against large lesions. Using nanoparticles to controllably generate heat in a localized manner may provide an alternative solution. Here we evaluate magnetic hyperthermia, using iron oxide magnetic nanoparticles, as a localized, heat-based method to kill the human-infective parasite in vitro. We assessed the effectiveness of this method against the differentiated, amastigote form of the parasite using three distinct viability assays: PrestoBlue, Live/Dead stain and a novel luciferase-based assay. Changes in amastigote morphology and ultrastructure were assessed by immunofluorescence, scanning and transmission electron microscopy. Our findings show that magnetic hyperthermia is an effective method to kill host-infective amastigotes, with morphological changes consistent with heat treatment. This method has the potential to be a step-change for research into new therapeutic options that moves away from the expensive chemotherapeutics currently dominating the research climate.",
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Nanoparticle-mediated magnetic hyperthermia is an effective method for killing the human-infective protozoan parasite Leishmania mexicana in vitro. / Berry, Sarah L.; Walker, Karen; Hoskins, Clare; Telling, Neil D.; Price, Helen P.

In: Scientific Reports, Vol. 9, 1059, 31.01.2019.

Research output: Contribution to journalArticle

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