Nanometrology

David J. S. Birch, Philip Yip

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

1 Citation (Scopus)

Abstract

Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
LanguageEnglish
Title of host publicationFluorescence Spectroscopy and Microscopy
Subtitle of host publicationMethods and Protocols
EditorsYves Engelborghs, Antonie Visser
Place of PublicationNew York
Pages279-302
Number of pages24
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

Fingerprint

nanoparticles
fluorescence
Einstein equations
metrology
colloids
dyes
silicon dioxide
anisotropy
decay

Keywords

  • fluorescence anisotropy
  • fluorescence lifetime
  • nanoparticle
  • silica
  • nanometrology
  • LUDOX®
  • sol–gel
  • TCSPC

Cite this

Birch, D. J. S., & Yip, P. (2014). Nanometrology. In Y. Engelborghs, & A. Visser (Eds.), Fluorescence Spectroscopy and Microscopy: Methods and Protocols (pp. 279-302). (Methods in Molecular Biology). New York. https://doi.org/10.1007/978-1-62703-649-8_11
Birch, David J. S. ; Yip, Philip. / Nanometrology. Fluorescence Spectroscopy and Microscopy: Methods and Protocols. editor / Yves Engelborghs ; Antonie Visser. New York, 2014. pp. 279-302 (Methods in Molecular Biology).
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keywords = "fluorescence anisotropy, fluorescence lifetime, nanoparticle, silica, nanometrology, LUDOX{\circledR}, sol–gel, TCSPC",
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Birch, DJS & Yip, P 2014, Nanometrology. in Y Engelborghs & A Visser (eds), Fluorescence Spectroscopy and Microscopy: Methods and Protocols. Methods in Molecular Biology, New York, pp. 279-302. https://doi.org/10.1007/978-1-62703-649-8_11

Nanometrology. / Birch, David J. S.; Yip, Philip.

Fluorescence Spectroscopy and Microscopy: Methods and Protocols. ed. / Yves Engelborghs; Antonie Visser. New York, 2014. p. 279-302 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

TY - CHAP

T1 - Nanometrology

AU - Birch, David J. S.

AU - Yip, Philip

N1 - The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-62703-649-8_11

PY - 2014

Y1 - 2014

N2 - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

AB - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

KW - fluorescence anisotropy

KW - fluorescence lifetime

KW - nanoparticle

KW - silica

KW - nanometrology

KW - LUDOX®

KW - sol–gel

KW - TCSPC

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DO - 10.1007/978-1-62703-649-8_11

M3 - Chapter (peer-reviewed)

SN - 9781627036481

T3 - Methods in Molecular Biology

SP - 279

EP - 302

BT - Fluorescence Spectroscopy and Microscopy

A2 - Engelborghs, Yves

A2 - Visser, Antonie

CY - New York

ER -

Birch DJS, Yip P. Nanometrology. In Engelborghs Y, Visser A, editors, Fluorescence Spectroscopy and Microscopy: Methods and Protocols. New York. 2014. p. 279-302. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-649-8_11