Nanometrology

David J. S. Birch, Philip Yip

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

3 Citations (Scopus)
117 Downloads (Pure)

Abstract

Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
Original languageEnglish
Title of host publicationFluorescence Spectroscopy and Microscopy
Subtitle of host publicationMethods and Protocols
EditorsYves Engelborghs, Antonie Visser
Place of PublicationNew York
Pages279-302
Number of pages24
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
PublisherHumana Press

Keywords

  • fluorescence anisotropy
  • fluorescence lifetime
  • nanoparticle
  • silica
  • nanometrology
  • LUDOX®
  • sol–gel
  • TCSPC

Student Theses

Nanometrology using time-resolved fluorescence techniques

Author: Yip, P., 8 Apr 2016

Supervisor: Birch, D. (Supervisor) & Chen, Y. (Supervisor)

Student thesis: Doctoral Thesis

Cite this

Birch, D. J. S., & Yip, P. (2014). Nanometrology. In Y. Engelborghs, & A. Visser (Eds.), Fluorescence Spectroscopy and Microscopy: Methods and Protocols (pp. 279-302). (Methods in Molecular Biology).. https://doi.org/10.1007/978-1-62703-649-8_11