Abstract
Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
| Original language | English |
|---|---|
| Title of host publication | Fluorescence Spectroscopy and Microscopy |
| Subtitle of host publication | Methods and Protocols |
| Editors | Yves Engelborghs, Antonie Visser |
| Place of Publication | New York |
| Pages | 279-302 |
| Number of pages | 24 |
| DOIs | |
| Publication status | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Publisher | Humana Press |
Keywords
- fluorescence anisotropy
- fluorescence lifetime
- nanoparticle
- silica
- nanometrology
- LUDOX®
- sol–gel
- TCSPC
Student Theses
Nanometrology using time-resolved fluorescence techniques
Author: Yip, P., 8 Apr 2016Supervisor: Birch, D. (Supervisor) & Chen, Y. (Supervisor)
Student thesis: Doctoral Thesis
Cite this
Birch, D. J. S., & Yip, P. (2014). Nanometrology. In Y. Engelborghs, & A. Visser (Eds.), Fluorescence Spectroscopy and Microscopy: Methods and Protocols (pp. 279-302). (Methods in Molecular Biology).. https://doi.org/10.1007/978-1-62703-649-8_11