Nanometrology

David J. S. Birch; Philip Yip

doi:10.1007/978-1-62703-649-8_11

Nanometrology

David J. S. Birch, Philip Yip

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

3 Citations (Scopus)

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Abstract

Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

Original language	English
Title of host publication	Fluorescence Spectroscopy and Microscopy
Subtitle of host publication	Methods and Protocols
Editors	Yves Engelborghs, Antonie Visser
Place of Publication	New York
Pages	279-302
Number of pages	24
DOIs	https://doi.org/10.1007/978-1-62703-649-8_11
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press

Keywords

fluorescence anisotropy
fluorescence lifetime
nanoparticle
silica
nanometrology
LUDOX®
sol–gel
TCSPC

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10.1007/978-1-62703-649-8_11

Brich-Yip-FSM2014-nanometrologyAccepted author manuscript, 493 KB

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title = "Nanometrology",

abstract = "Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.",

keywords = "fluorescence anisotropy, fluorescence lifetime, nanoparticle, silica, nanometrology, LUDOX{\textregistered}, sol–gel, TCSPC",

author = "Birch, {David J. S.} and Philip Yip",

note = "The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-62703-649-8_11",

year = "2014",

doi = "10.1007/978-1-62703-649-8_11",

language = "English",

isbn = "9781627036481",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "279--302",

editor = "Yves Engelborghs and Antonie Visser",

booktitle = "Fluorescence Spectroscopy and Microscopy",

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TY - CHAP

T1 - Nanometrology

AU - Birch, David J. S.

AU - Yip, Philip

N1 - The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-62703-649-8_11

PY - 2014

Y1 - 2014

N2 - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

AB - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.

KW - fluorescence anisotropy

KW - fluorescence lifetime

KW - nanoparticle

KW - silica

KW - nanometrology

KW - LUDOX®

KW - sol–gel

KW - TCSPC

UR - http://link.springer.com/book/10.1007/978-1-62703-649-8

UR - http://www.springer.com/

U2 - 10.1007/978-1-62703-649-8_11

DO - 10.1007/978-1-62703-649-8_11

M3 - Chapter (peer-reviewed)

SN - 9781627036481

T3 - Methods in Molecular Biology

SP - 279

EP - 302

BT - Fluorescence Spectroscopy and Microscopy

A2 - Engelborghs, Yves

A2 - Visser, Antonie

CY - New York

ER -

Nanometrology

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Nanometrology using time-resolved fluorescence techniques

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Nanometrology

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Student Theses

Nanometrology using time-resolved fluorescence techniques

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