Abstract
| Original language | English |
|---|---|
| Title of host publication | Fluorescence Spectroscopy and Microscopy |
| Subtitle of host publication | Methods and Protocols |
| Editors | Yves Engelborghs, Antonie Visser |
| Place of Publication | New York |
| Pages | 279-302 |
| Number of pages | 24 |
| DOIs | |
| Publication status | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Publisher | Humana Press |
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Keywords
- fluorescence anisotropy
- fluorescence lifetime
- nanoparticle
- silica
- nanometrology
- LUDOX®
- sol–gel
- TCSPC
Cite this
}
Nanometrology. / Birch, David J. S.; Yip, Philip.
Fluorescence Spectroscopy and Microscopy: Methods and Protocols. ed. / Yves Engelborghs; Antonie Visser. New York, 2014. p. 279-302 (Methods in Molecular Biology).Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed)
TY - CHAP
T1 - Nanometrology
AU - Birch, David J. S.
AU - Yip, Philip
N1 - The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-62703-649-8_11
PY - 2014
Y1 - 2014
N2 - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
AB - Methods and protocols are described when using fluorescence metrology to determine the average nanoparticle (np) size in colloids in the range of 1–10 nm. The technique is based on determining the rotational correlation time of the np from the decay of fluorescence anisotropy of a dye that is electrostatically or covalently attached to the np as it undergoes Brownian rotation. The np size is then calculated from the Stokes–Einstein equation. The exemplar of silica nps is presented, but the approach can also be applied to other types of nps.
KW - fluorescence anisotropy
KW - fluorescence lifetime
KW - nanoparticle
KW - silica
KW - nanometrology
KW - LUDOX®
KW - sol–gel
KW - TCSPC
UR - http://link.springer.com/book/10.1007/978-1-62703-649-8
UR - http://www.springer.com/
U2 - 10.1007/978-1-62703-649-8_11
DO - 10.1007/978-1-62703-649-8_11
M3 - Chapter (peer-reviewed)
SN - 9781627036481
T3 - Methods in Molecular Biology
SP - 279
EP - 302
BT - Fluorescence Spectroscopy and Microscopy
A2 - Engelborghs, Yves
A2 - Visser, Antonie
CY - New York
ER -