Naked-eye detection as a universal approach to lower the limit of detection of enzyme-linked immunoassays

Erin F. O'Connor, Sureyya Paterson, Roberto De La Rica

Research output: Contribution to journalLetter

9 Citations (Scopus)

Abstract

Colorimetric biosensors for the detection of analytes with the naked eye are required in environmental monitoring, point-of-care diagnostics, and analyses in resources constrained settings, where detection instruments may not be available. However instrument-based detection methods are usually more adequate for detecting small variations in the signal compared to naked-eye detection schemes, and consequently the limit of detection of the latter is usually higher than the former. Here we demonstrate that the limit of detection of colorimetric enzyme-linked immunoassays can be decreased several orders of magnitude when using naked-eye detection instead of a spectrophotometer for detecting the signal. The key step to lower the limit of detection is adding a small volume of chromogenic substrate during the signal generation step. This generates highly colored solutions that can be easily visualized with the naked eye and recorded with the camera of a mobile phone. The proposed method does not require expensive equipment or complex protocols to enhance the
signal, and therefore it is a universal approach to lower the limit of detection of
colorimetric enzyme-linked immunoassays.
LanguageEnglish
Pages3389-3393
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number13
Early online date12 Mar 2016
DOIs
Publication statusPublished - 12 May 2016

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Immunoenzyme Techniques
Limit of Detection
Chromogenic Compounds
Spectrophotometers
Enzymes
Mobile phones
Biosensors
Cameras
Point-of-Care Systems
Cell Phones
Monitoring
Environmental Monitoring
Biosensing Techniques
Equipment and Supplies

Keywords

  • ELISA
  • antibody
  • biosensor
  • immunosensor
  • smartphone
  • naked-eye detection

Cite this

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title = "Naked-eye detection as a universal approach to lower the limit of detection of enzyme-linked immunoassays",
abstract = "Colorimetric biosensors for the detection of analytes with the naked eye are required in environmental monitoring, point-of-care diagnostics, and analyses in resources constrained settings, where detection instruments may not be available. However instrument-based detection methods are usually more adequate for detecting small variations in the signal compared to naked-eye detection schemes, and consequently the limit of detection of the latter is usually higher than the former. Here we demonstrate that the limit of detection of colorimetric enzyme-linked immunoassays can be decreased several orders of magnitude when using naked-eye detection instead of a spectrophotometer for detecting the signal. The key step to lower the limit of detection is adding a small volume of chromogenic substrate during the signal generation step. This generates highly colored solutions that can be easily visualized with the naked eye and recorded with the camera of a mobile phone. The proposed method does not require expensive equipment or complex protocols to enhance thesignal, and therefore it is a universal approach to lower the limit of detection ofcolorimetric enzyme-linked immunoassays.",
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Naked-eye detection as a universal approach to lower the limit of detection of enzyme-linked immunoassays. / O'Connor, Erin F.; Paterson, Sureyya; De La Rica, Roberto.

In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 13, 12.05.2016, p. 3389-3393.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Naked-eye detection as a universal approach to lower the limit of detection of enzyme-linked immunoassays

AU - O'Connor, Erin F.

AU - Paterson, Sureyya

AU - De La Rica, Roberto

PY - 2016/5/12

Y1 - 2016/5/12

N2 - Colorimetric biosensors for the detection of analytes with the naked eye are required in environmental monitoring, point-of-care diagnostics, and analyses in resources constrained settings, where detection instruments may not be available. However instrument-based detection methods are usually more adequate for detecting small variations in the signal compared to naked-eye detection schemes, and consequently the limit of detection of the latter is usually higher than the former. Here we demonstrate that the limit of detection of colorimetric enzyme-linked immunoassays can be decreased several orders of magnitude when using naked-eye detection instead of a spectrophotometer for detecting the signal. The key step to lower the limit of detection is adding a small volume of chromogenic substrate during the signal generation step. This generates highly colored solutions that can be easily visualized with the naked eye and recorded with the camera of a mobile phone. The proposed method does not require expensive equipment or complex protocols to enhance thesignal, and therefore it is a universal approach to lower the limit of detection ofcolorimetric enzyme-linked immunoassays.

AB - Colorimetric biosensors for the detection of analytes with the naked eye are required in environmental monitoring, point-of-care diagnostics, and analyses in resources constrained settings, where detection instruments may not be available. However instrument-based detection methods are usually more adequate for detecting small variations in the signal compared to naked-eye detection schemes, and consequently the limit of detection of the latter is usually higher than the former. Here we demonstrate that the limit of detection of colorimetric enzyme-linked immunoassays can be decreased several orders of magnitude when using naked-eye detection instead of a spectrophotometer for detecting the signal. The key step to lower the limit of detection is adding a small volume of chromogenic substrate during the signal generation step. This generates highly colored solutions that can be easily visualized with the naked eye and recorded with the camera of a mobile phone. The proposed method does not require expensive equipment or complex protocols to enhance thesignal, and therefore it is a universal approach to lower the limit of detection ofcolorimetric enzyme-linked immunoassays.

KW - ELISA

KW - antibody

KW - biosensor

KW - immunosensor

KW - smartphone

KW - naked-eye detection

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DO - 10.1007/s00216-016-9453-8

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JO - Analytical and Bioanalytical Chemistry

T2 - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

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