Naive t-cell receptor transgenic t cells help memory b cells produce antibody

D. Duffy, C.P. Yang, A. Heath, P. Garside, E.B. Bell

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Injection of the same antigen following primary immunization induces a classic secondary response characterized by a large quantity of high-affinity antibody of an immunoglobulin G class produced more rapidly than in the initial response – the products of memory B cells are qualitatively distinct from that of the original naive B lymphocytes. Very little is known of the help provided by the CD4 T cells that stimulate memory B cells. Using antigen-specific T-cell receptor transgenic CD4 T cells (DO11.10) as a source of help, we found that naive transgenic T cells stimulated memory B cells almost as well (in terms of quantity and speed) as transgenic T cells that had been recently primed. There was a direct correlation between serum antibody levels and the number of naive transgenic T cells transferred. Using T cells from transgenic interleukin-2-deficient mice we showed that interleukin-2 was not required for a secondary response, although it was necessary for a primary response. The results suggested that the signals delivered by CD4 T cells and required by memory B cells for their activation were common to both antigen-primed and naive CD4 T cells.

Original languageEnglish
Pages (from-to)376-384
Number of pages9
Publication statusPublished - 2006


  • adoptive transfer
  • transgenic
  • memory
  • CD45RB
  • CD4 T cells


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