NADPH as a potential intrinsic probe for tumour margin estimation

Hazel L. Stewart, Ted R. Hupp, David J. S. Birch

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.
LanguageEnglish
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationModules, Devices, and Applications IV
EditorsBrian W. Pogue, Sylvain Gioux, Gregg Biggs
Place of PublicationBellingham, WA
Number of pages12
DOIs
Publication statusPublished - 1 Mar 2018
EventSPIE Photonics West 2018 BIOS - Moscone Centre , San Francisco, United States
Duration: 27 Jan 20181 Feb 2018

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10478

Conference

ConferenceSPIE Photonics West 2018 BIOS
CountryUnited States
CitySan Francisco
Period27/01/181/02/18

Fingerprint

margins
tumors
life (durability)
fluorescence
probes
cancer
surgery
coenzymes
biosynthesis
oxygen
enzymes
counting
silicon dioxide
filters
photons
liquids
cells

Keywords

  • fluorescence lifetime
  • NADPH
  • fluorescence spectroscopy
  • tumour margin estimation
  • intrinsic probe
  • liquid light guide

Cite this

Stewart, H. L., Hupp, T. R., & Birch, D. J. S. (2018). NADPH as a potential intrinsic probe for tumour margin estimation. In B. W. Pogue, S. Gioux, & G. Biggs (Eds.), Molecular-Guided Surgery: Modules, Devices, and Applications IV [1047817] (Proceedings of SPIE; Vol. 10478). Bellingham, WA. https://doi.org/10.1117/12.2287414
Stewart, Hazel L. ; Hupp, Ted R. ; Birch, David J. S. / NADPH as a potential intrinsic probe for tumour margin estimation. Molecular-Guided Surgery: Modules, Devices, and Applications IV. editor / Brian W. Pogue ; Sylvain Gioux ; Gregg Biggs. Bellingham, WA, 2018. (Proceedings of SPIE).
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Stewart, HL, Hupp, TR & Birch, DJS 2018, NADPH as a potential intrinsic probe for tumour margin estimation. in BW Pogue, S Gioux & G Biggs (eds), Molecular-Guided Surgery: Modules, Devices, and Applications IV., 1047817, Proceedings of SPIE, vol. 10478, Bellingham, WA, SPIE Photonics West 2018 BIOS, San Francisco, United States, 27/01/18. https://doi.org/10.1117/12.2287414

NADPH as a potential intrinsic probe for tumour margin estimation. / Stewart, Hazel L.; Hupp, Ted R.; Birch, David J. S.

Molecular-Guided Surgery: Modules, Devices, and Applications IV. ed. / Brian W. Pogue; Sylvain Gioux; Gregg Biggs. Bellingham, WA, 2018. 1047817 (Proceedings of SPIE; Vol. 10478).

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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N2 - The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

AB - The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

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Stewart HL, Hupp TR, Birch DJS. NADPH as a potential intrinsic probe for tumour margin estimation. In Pogue BW, Gioux S, Biggs G, editors, Molecular-Guided Surgery: Modules, Devices, and Applications IV. Bellingham, WA. 2018. 1047817. (Proceedings of SPIE). https://doi.org/10.1117/12.2287414