NADPH as a potential intrinsic probe for tumour margin estimation

Hazel L. Stewart; Ted R. Hupp; David J. S. Birch

doi:10.1117/12.2287414

NADPH as a potential intrinsic probe for tumour margin estimation

Hazel L. Stewart, Ted R. Hupp, David J. S. Birch

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

53 Downloads (Pure)

Abstract

The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

Original language	English
Title of host publication	Molecular-Guided Surgery
Subtitle of host publication	Modules, Devices, and Applications IV
Editors	Brian W. Pogue, Sylvain Gioux, Gregg Biggs
Place of Publication	Bellingham, WA
Number of pages	12
DOIs	https://doi.org/10.1117/12.2287414
Publication status	Published - 1 Mar 2018
Event	SPIE Photonics West 2018: OPTO - The Moscone Center, San Francisco, United States Duration: 27 Jan 2018 → 1 Feb 2018 http://spie.org/conferences-and-exhibitions/photonics-west/?SSO=1

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	10478

Conference

Conference	SPIE Photonics West 2018
Abbreviated title	SPIE Photonics West 2018
Country/Territory	United States
City	San Francisco
Period	27/01/18 → 1/02/18
Internet address	http://spie.org/conferences-and-exhibitions/photonics-west/?SSO=1

Keywords

fluorescence lifetime
NADPH
fluorescence spectroscopy
tumour margin estimation
intrinsic probe
liquid light guide

Access to Document

10.1117/12.2287414

Stewart-etal-BIOS2018-NADPH-as-a-potential-intrinsic-probe-for-tumour-marginAccepted author manuscript, 1.29 MB

2 Keynote
1 Review article

Time will tell in fluorescence guided surgery
Birch, D. & Stewart, H. L., 9 Sept 2024.
Research output: Contribution to conference › Keynote › peer-review
Emerging clinical applications of fluorescence using SPADs
Birch, D., 15 Jun 2022.
Research output: Contribution to conference › Keynote
Fluorescence guided surgery
Stewart, H. L. & Birch, D. J. S., 26 Aug 2021, In: Methods and Applications in Fluorescence . 9, 4, 26 p., 042002.
Research output: Contribution to journal › Review article › peer-review

Open Access
File
54 Citations (Scopus)

23 Downloads (Pure)

Cite this

@inproceedings{05be0b5bb695485283d808dfcad5f44e,

title = "NADPH as a potential intrinsic probe for tumour margin estimation",

abstract = "The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments. ",

keywords = "fluorescence lifetime, NADPH, fluorescence spectroscopy, tumour margin estimation, intrinsic probe, liquid light guide",

author = "Stewart, {Hazel L.} and Hupp, {Ted R.} and Birch, {David J. S.}",

note = "Hazel Stewart, Ted R. Hupp, David J. S. Birch, {"}NADPH as a potential intrinsic probe for tumour margin estimation{"}, Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 1047817 (1 March 2018); doi: 10.1117/12.2287414. {\textcopyright} 2018 Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited. ; SPIE Photonics West 2018 : OPTO, SPIE Photonics West 2018 ; Conference date: 27-01-2018 Through 01-02-2018",

year = "2018",

month = mar,

day = "1",

doi = "10.1117/12.2287414",

language = "English",

series = "Proceedings of SPIE",

publisher = "SPIE",

editor = "Pogue, {Brian W.} and Sylvain Gioux and Gregg Biggs",

booktitle = "Molecular-Guided Surgery",

url = "http://spie.org/conferences-and-exhibitions/photonics-west/?SSO=1",

}

Stewart, HL, Hupp, TR & Birch, DJS 2018, NADPH as a potential intrinsic probe for tumour margin estimation. in BW Pogue, S Gioux & G Biggs (eds), Molecular-Guided Surgery: Modules, Devices, and Applications IV., 1047817, Proceedings of SPIE, vol. 10478, Bellingham, WA, SPIE Photonics West 2018, San Francisco, California, United States, 27/01/18. https://doi.org/10.1117/12.2287414

NADPH as a potential intrinsic probe for tumour margin estimation. / Stewart, Hazel L.; Hupp, Ted R.; Birch, David J. S.
Molecular-Guided Surgery: Modules, Devices, and Applications IV. ed. / Brian W. Pogue; Sylvain Gioux; Gregg Biggs. Bellingham, WA, 2018. 1047817 (Proceedings of SPIE; Vol. 10478).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

TY - GEN

T1 - NADPH as a potential intrinsic probe for tumour margin estimation

AU - Stewart, Hazel L.

AU - Hupp, Ted R.

AU - Birch, David J. S.

N1 - Hazel Stewart, Ted R. Hupp, David J. S. Birch, "NADPH as a potential intrinsic probe for tumour margin estimation", Proc. SPIE 10478, Molecular-Guided Surgery: Molecules, Devices, and Applications IV, 1047817 (1 March 2018); doi: 10.1117/12.2287414. © 2018 Society of Photo Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, or modification of the contents of the publication are prohibited.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

AB - The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.

KW - fluorescence lifetime

KW - NADPH

KW - fluorescence spectroscopy

KW - tumour margin estimation

KW - intrinsic probe

KW - liquid light guide

U2 - 10.1117/12.2287414

DO - 10.1117/12.2287414

M3 - Conference contribution book

T3 - Proceedings of SPIE

BT - Molecular-Guided Surgery

A2 - Pogue, Brian W.

A2 - Gioux, Sylvain

A2 - Biggs, Gregg

CY - Bellingham, WA

T2 - SPIE Photonics West 2018

Y2 - 27 January 2018 through 1 February 2018

ER -

NADPH as a potential intrinsic probe for tumour margin estimation

Abstract

Publication series

Conference

Keywords

Access to Document

Fingerprint

Research output

Time will tell in fluorescence guided surgery

Emerging clinical applications of fluorescence using SPADs

Fluorescence guided surgery

Cite this