NADPH as a potential intrinsic probe for tumour margin estimation

Hazel L. Stewart, Ted R. Hupp, David J. S. Birch

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Abstract

The fluorescent properties of the reduced coenzyme NADH and its phosphorylated derivative (NADPH) have been explored in order to assess their potential as an intrinsic probe for cancer surgery. NADPH production is increased in cancer cells to quench reactive oxygen species and meet higher demands for biosynthesis, and has attractive fluorescent properties such as emission towards the visible part of the spectrum and a relatively long fluorescence lifetime upon binding to enzymes (~ 1 – 6.5 ns) that helps discriminate against other endogenous species. Different environmental effects on NAD(P)H fluorescence are reported here, including an increase in lifetime upon oxygen removal, an ability to retain its fluorescent properties in a complex medium (a silica phantom) and its fluorescence lifetime also being distinguishable in a cell environment. In addition, the development of a miniaturized liquid light guide filter-based timecorrelated single photon counting fluorescence lifetime system is reported as a step towards time-resolved visual imaging in cancer surgery. This system has been demonstrated as being capable of accurately measuring NAD(P)H fluorescence lifetimes in both simple solvent and cellular environments.
Original languageEnglish
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationModules, Devices, and Applications IV
EditorsBrian W. Pogue, Sylvain Gioux, Gregg Biggs
Place of PublicationBellingham, WA
Number of pages12
DOIs
Publication statusPublished - 1 Mar 2018
EventSPIE Photonics West 2018: OPTO - The Moscone Center, San Francisco, United States
Duration: 27 Jan 20181 Feb 2018
http://spie.org/conferences-and-exhibitions/photonics-west/?SSO=1

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10478

Conference

ConferenceSPIE Photonics West 2018
Abbreviated titleSPIE Photonics West 2018
CountryUnited States
CitySan Francisco
Period27/01/181/02/18
Internet address

Keywords

  • fluorescence lifetime
  • NADPH
  • fluorescence spectroscopy
  • tumour margin estimation
  • intrinsic probe
  • liquid light guide

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