Muscarinic toxin selective for m4 receptors impairs memory in the rat

Diana Jerusalinsky, Edgar Kornisiuk, Paula Alfaro, Jorge Quillfeldt, Mariana Alonso, Emiliano Rial Verde, Carlos Cerveñansky, Alan Harvey

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The selectivity of the muscarinic toxin MT3 from green mamba snake venom was corroborated by inhibition of the binding of [3H]NMS, a classical muscarinic radioligand, to native and cloned muscarinic receptors, showing 214-fold higher affinity for m4 than for m1 subtype, without significant binding to the others. The highest concentrations of MT3 sites (putative m4 receptors) in the rat brain were found in striatum and olfactory tubercle, intermediate concentration in dentate gyrus and CA1, and lower but still conspicuous levels in CA3 and frontal cortex. MT3 caused retrograde amnesia of an inhibitory avoidance task, when injected into the dorsal hippocampus of rats after training, suggesting a positive role of these MT3 sensitive sites, which are probably m4 muscarinic receptors, in memory consolidation of this task.

LanguageEnglish
Pages1407-1411
Number of pages5
JournalNeuroReport
Volume9
Issue number7
DOIs
Publication statusPublished - 11 May 1998

Fingerprint

Cholinergic Agents
Muscarinic M4 Receptors
Retrograde Amnesia
Snake Venoms
Dentate Gyrus
Frontal Lobe
Muscarinic Receptors
Hippocampus
Brain
Inhibition (Psychology)
Olfactory Tubercle
Memory Consolidation
muscarinic toxin 3

Keywords

  • memory
  • muscarinic m4 receptors
  • muscarinic receptors
  • muscarinic toxin

Cite this

Jerusalinsky, D., Kornisiuk, E., Alfaro, P., Quillfeldt, J., Alonso, M., Verde, E. R., ... Harvey, A. (1998). Muscarinic toxin selective for m4 receptors impairs memory in the rat. NeuroReport, 9(7), 1407-1411. https://doi.org/10.1097/00001756-199805110-00029
Jerusalinsky, Diana ; Kornisiuk, Edgar ; Alfaro, Paula ; Quillfeldt, Jorge ; Alonso, Mariana ; Verde, Emiliano Rial ; Cerveñansky, Carlos ; Harvey, Alan. / Muscarinic toxin selective for m4 receptors impairs memory in the rat. In: NeuroReport. 1998 ; Vol. 9, No. 7. pp. 1407-1411.
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Jerusalinsky, D, Kornisiuk, E, Alfaro, P, Quillfeldt, J, Alonso, M, Verde, ER, Cerveñansky, C & Harvey, A 1998, 'Muscarinic toxin selective for m4 receptors impairs memory in the rat' NeuroReport, vol. 9, no. 7, pp. 1407-1411. https://doi.org/10.1097/00001756-199805110-00029

Muscarinic toxin selective for m4 receptors impairs memory in the rat. / Jerusalinsky, Diana; Kornisiuk, Edgar; Alfaro, Paula; Quillfeldt, Jorge; Alonso, Mariana; Verde, Emiliano Rial; Cerveñansky, Carlos; Harvey, Alan.

In: NeuroReport, Vol. 9, No. 7, 11.05.1998, p. 1407-1411.

Research output: Contribution to journalArticle

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Jerusalinsky D, Kornisiuk E, Alfaro P, Quillfeldt J, Alonso M, Verde ER et al. Muscarinic toxin selective for m4 receptors impairs memory in the rat. NeuroReport. 1998 May 11;9(7):1407-1411. https://doi.org/10.1097/00001756-199805110-00029