Murine neutrophils present Class II restricted antigen

S. Culshaw, O.R. Millington, J.M. Brewer, I.B. McInnes

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Neutrophils were originally described as short lived, terminally differentiated phagocytes that contribute only to the innate immune response. Recent evidence of neutrophil cytokine production and expression of numerous cell surface proteins has suggested that neutrophils are likely to influence adaptive responses and may satisfy the criteria of antigen presenting cells. Under certain inflammatory conditions human neutrophils express major histocompatibilty complex (MHC) Class II and the costimulatory molecules CD80 and CD86. We have employed a murine T cell hybridoma with a transgenic T cell receptor specific for ovalbumin peptide 323-339 (OVA323-339), and a green fluorescent reporter of T cell receptor ligation, to directly investigate neutrophil-T cell interactions. These cells provide an ideal model system, allowing precise identification of antigen specificity and a clear readout of T cell activation. Additionally, whilst murine neutrophils have previously been shown to stimulate MHC Class I-dependent CD8+ T cell activation, CD4+ T cells stimulation via MHC Class II-expressing neutrophils has not been investigated. We addressed this by isolating murine neutrophils, loading with OVA323-339 and co-culturing with T cells specific for the OVA323-339/MHC Class II complex, and this resulted in T cell activation, as determined by expression of the green-fluorescent protein reporter. Antigen-pulsed neutrophils were also able to prime naïve OVA-specific CD4+ T cells in a contact-dependent manner, as shown by proliferation and cytokine production. Activation of lymphocytes was not due to contaminating macrophages. These studies demonstrate that murine neutrophils present MHC Class II-restricted peptides and induce T cell proliferation, confirming findings in human neutrophils, and demonstrate a novel pro inflammatory effect of murine neutrophils.
LanguageEnglish
Pages49-54
Number of pages6
JournalImmunology Letters
Volume118
Issue number1
DOIs
Publication statusPublished - 15 Jun 2008

Fingerprint

Histocompatibility Antigens Class II
Neutrophils
T-Lymphocytes
T-Cell Antigen Receptor
Cytokines
Peptide T
Antigens
Hybridomas
Antigen-Presenting Cells
Phagocytes
Lymphocyte Activation
Green Fluorescent Proteins
Innate Immunity
Cell Communication
Ligation
Membrane Proteins
Macrophages
Cell Proliferation

Keywords

  • murine
  • neutrophil
  • antigen presentation
  • cells
  • biophotonics

Cite this

Culshaw, S., Millington, O. R., Brewer, J. M., & McInnes, I. B. (2008). Murine neutrophils present Class II restricted antigen. Immunology Letters, 118(1), 49-54. https://doi.org/10.1016/j.imlet.2008.02.008
Culshaw, S. ; Millington, O.R. ; Brewer, J.M. ; McInnes, I.B. / Murine neutrophils present Class II restricted antigen. In: Immunology Letters. 2008 ; Vol. 118, No. 1. pp. 49-54.
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Culshaw, S, Millington, OR, Brewer, JM & McInnes, IB 2008, 'Murine neutrophils present Class II restricted antigen' Immunology Letters, vol. 118, no. 1, pp. 49-54. https://doi.org/10.1016/j.imlet.2008.02.008

Murine neutrophils present Class II restricted antigen. / Culshaw, S.; Millington, O.R.; Brewer, J.M.; McInnes, I.B.

In: Immunology Letters, Vol. 118, No. 1, 15.06.2008, p. 49-54.

Research output: Contribution to journalArticle

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Culshaw S, Millington OR, Brewer JM, McInnes IB. Murine neutrophils present Class II restricted antigen. Immunology Letters. 2008 Jun 15;118(1):49-54. https://doi.org/10.1016/j.imlet.2008.02.008