Multivariate data analysis and metabolic profiling of artemisinin and related compounds in high yielding varieties of Artemisia annua field-grown in Madagascar

John Suberu, Piotr S. Gromski, Alison Nordon, Alexei Lapkin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol for rapid analysis of co-metabolites of A. annua in raw extracts was developed and extensively characterized. The new method was used to analyse metabolic profiles of 13 varieties of A. annua from an in-field growth programme in Madagascar. Several multivariate data analysis techniques consistently show the association of artemisinin with dihydroartemisinic acid. These data support the hypothesis of dihydroartemisinic acid being the late stage precursor to artemisinin in its biosynthetic pathway.

LanguageEnglish
Pages522-531
Number of pages10
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume117
DOIs
Publication statusPublished - 5 Jan 2016

Fingerprint

Artemisia annua
Madagascar
Multivariate Analysis
Metabolome
Biosynthetic Pathways
Liquid chromatography
Metabolites
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Association reactions
Growth
artemisinine
dihydroartemisinic acid

Keywords

  • Artemisia annua
  • metabolic profiling
  • multivariate data analysis

Cite this

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AU - Nordon, Alison

AU - Lapkin, Alexei

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AB - An improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) protocol for rapid analysis of co-metabolites of A. annua in raw extracts was developed and extensively characterized. The new method was used to analyse metabolic profiles of 13 varieties of A. annua from an in-field growth programme in Madagascar. Several multivariate data analysis techniques consistently show the association of artemisinin with dihydroartemisinic acid. These data support the hypothesis of dihydroartemisinic acid being the late stage precursor to artemisinin in its biosynthetic pathway.

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