Multiplexed detection methods for profiling microRNA expression in biological samples

A.W. Wark, Hye Jin Lee, R.M. Corn

Research output: Contribution to journalLiterature review

205 Citations (Scopus)

Abstract

The recent discovery of short, non-protein coding RNA molecules, such as microRNA molecules (miRNAs), that can control gene expression has unveiled a whole new layer of complexity in the regulation of cell function. Since 2001, there has been a surge of interest in understanding the regulatory role of the hundreds to thousands of miRNAs expressed in both plants and animals. Significant progress in this area requires the development of quantitative bioanalytical methods for the rapid, multiplexed detection of all miRNAs that are present in a particular cell or tissue sample. In this Minireview, we discuss some of the latest methods for high-throughput miRNA profiling and the unique technological challenges that must be surmounted in this endeavor.
Original languageEnglish
Pages (from-to)644-652
Number of pages9
JournalAngewandte Chemie
Volume47
Issue number4
Early online date12 Nov 2007
DOIs
Publication statusPublished - Jan 2008

Keywords

  • gene expression
  • microarrays
  • microRNA
  • RNA profiling
  • RNA silencing

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