Multifunctional silk-heparin biomaterials for vascular tissue engineering applications

Friedrich Philipp Seib, Manueal Herklotz, Kelly Burke, Manfred Maitz, Carsten Werner, David Kaplan

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.
LanguageEnglish
Pages83-91
Number of pages9
JournalBiomaterials
Volume35
Issue number1
Early online date4 Oct 2013
DOIs
Publication statusPublished - 1 Jan 2014

Fingerprint

Silk
Biocompatible Materials
Tissue Engineering
Tissue engineering
Biomaterials
Blood Vessels
Heparin
Vascular Endothelial Growth Factor A
Endothelial cells
Sutures
Endothelial Cells
Materials Testing
Complement Activation
Low Molecular Weight Heparin
Polytetrafluoroethylene
Biocompatibility
Polytetrafluoroethylenes
Anticoagulants
Refining
Endothelium

Keywords

  • endothelial cells
  • heparin
  • blood compatibility
  • silk
  • VEGF
  • vascular engineering
  • silk-heparin biomaterials
  • vascular tissue engineering

Cite this

Seib, Friedrich Philipp ; Herklotz, Manueal ; Burke, Kelly ; Maitz, Manfred ; Werner, Carsten ; Kaplan, David. / Multifunctional silk-heparin biomaterials for vascular tissue engineering applications. In: Biomaterials. 2014 ; Vol. 35, No. 1. pp. 83-91.
@article{fa4f390214e04c9196df9c240119f68f,
title = "Multifunctional silk-heparin biomaterials for vascular tissue engineering applications",
abstract = "Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.",
keywords = "endothelial cells, heparin, blood compatibility, silk, VEGF, vascular engineering, silk-heparin biomaterials, vascular tissue engineering",
author = "Seib, {Friedrich Philipp} and Manueal Herklotz and Kelly Burke and Manfred Maitz and Carsten Werner and David Kaplan",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2013.09.053",
language = "English",
volume = "35",
pages = "83--91",
journal = "Biomaterials",
issn = "0142-9612",
number = "1",

}

Multifunctional silk-heparin biomaterials for vascular tissue engineering applications. / Seib, Friedrich Philipp; Herklotz, Manueal; Burke, Kelly; Maitz, Manfred; Werner, Carsten; Kaplan, David.

In: Biomaterials, Vol. 35, No. 1, 01.01.2014, p. 83-91.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multifunctional silk-heparin biomaterials for vascular tissue engineering applications

AU - Seib, Friedrich Philipp

AU - Herklotz, Manueal

AU - Burke, Kelly

AU - Maitz, Manfred

AU - Werner, Carsten

AU - Kaplan, David

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.

AB - Over the past 30 years, silk has been proposed for numerous biomedical applications that go beyond its traditional use as a suture material. Silk sutures are well tolerated in humans, but the use of silk for vascular engineering applications still requires extensive biocompatibility testing. Some studies have indicated a need to modify silk to yield a hemocompatible surface. This study examined the potential of low molecular weight heparin as a material for refining silk properties by acting as a carrier for vascular endothelial growth factor (VEGF) and improving silk hemocompatibility. Heparinized silk showed a controlled VEGF release over 6 days; the released VEGF was bioactive and supported the growth of human endothelial cells. Silk samples were then assessed using a humanized hemocompatibility system that employs whole blood and endothelial cells. The overall thrombogenic response for silk was very low and similar to the clinical reference material polytetrafluoroethylene. Despite an initial inflammatory response to silk, apparent as complement and leukocyte activation, the endothelium was maintained in a resting, anticoagulant state. The low thrombogenic response and the ability to control VEGF release support the further development of silk for vascular applications.

KW - endothelial cells

KW - heparin

KW - blood compatibility

KW - silk

KW - VEGF

KW - vascular engineering

KW - silk-heparin biomaterials

KW - vascular tissue engineering

UR - http://www.ncbi.nlm.nih.gov/pubmed/24099708

U2 - 10.1016/j.biomaterials.2013.09.053

DO - 10.1016/j.biomaterials.2013.09.053

M3 - Article

VL - 35

SP - 83

EP - 91

JO - Biomaterials

T2 - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 1

ER -