Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to chronic myeloid leukemia

Pedro Urbano Alves, Raquel Vinhas, Alexandra R. Fernandes, Semra Zuhal Birol, Levent Trabzon, Iwona Bernacka-Wojcik, Rui Igreja, Paulo Lopes, Pedro Viana Baptista, Hugo Águas, Elvira Fortunato, Rodrigo Martins

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.
LanguageEnglish
Article number381
Number of pages10
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 10 Jan 2018

Fingerprint

Nanoprobes
leukemias
RNA
Microfluidics
Screening
chips
screening
Gold
gold
Amplification
Optical fibers
Signal to noise ratio
Health
microbalances
health
signal to noise ratios
sectors
optical fibers
costs
Costs

Keywords

  • assay systems
  • biomedical engineering
  • imaging and sensing
  • sensors and biosensors

Cite this

Alves, Pedro Urbano ; Vinhas, Raquel ; Fernandes, Alexandra R. ; Birol, Semra Zuhal ; Trabzon, Levent ; Bernacka-Wojcik, Iwona ; Igreja, Rui ; Lopes, Paulo ; Baptista, Pedro Viana ; Águas, Hugo ; Fortunato, Elvira ; Martins, Rodrigo. / Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to chronic myeloid leukemia. In: Scientific Reports. 2018 ; Vol. 8.
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abstract = "Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.",
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Alves, PU, Vinhas, R, Fernandes, AR, Birol, SZ, Trabzon, L, Bernacka-Wojcik, I, Igreja, R, Lopes, P, Baptista, PV, Águas, H, Fortunato, E & Martins, R 2018, 'Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to chronic myeloid leukemia' Scientific Reports, vol. 8, 381. https://doi.org/10.1038/s41598-017-18725-9

Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to chronic myeloid leukemia. / Alves, Pedro Urbano; Vinhas, Raquel; Fernandes, Alexandra R.; Birol, Semra Zuhal; Trabzon, Levent; Bernacka-Wojcik, Iwona; Igreja, Rui; Lopes, Paulo; Baptista, Pedro Viana; Águas, Hugo; Fortunato, Elvira; Martins, Rodrigo.

In: Scientific Reports, Vol. 8, 381, 10.01.2018.

Research output: Contribution to journalArticle

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AU - Alves, Pedro Urbano

AU - Vinhas, Raquel

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AU - Birol, Semra Zuhal

AU - Trabzon, Levent

AU - Bernacka-Wojcik, Iwona

AU - Igreja, Rui

AU - Lopes, Paulo

AU - Baptista, Pedro Viana

AU - Águas, Hugo

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AU - Martins, Rodrigo

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N2 - Many diseases have their treatment options narrowed and end up being fatal if detected during later stages. As a consequence, point-of-care devices have an increasing importance for routine screening applications in the health sector due to their portability, fast analyses and decreased cost. For that purpose, a multifunctional chip was developed and tested using gold nanoprobes to perform RNA optical detection inside a microfluidic chip without the need of molecular amplification steps. As a proof-of-concept, this device was used for the rapid detection of chronic myeloid leukemia, a hemato-oncological disease that would benefit from early stage diagnostics and screening tests. The chip passively mixed target RNA from samples, gold nanoprobes and saline solution to infer a result from their final colorimetric properties. An optical fiber network was used to evaluate its transmitted spectra inside the chip. Trials provided accurate output results within 3 min, yielding signal-to-noise ratios up to 9 dB. When compared to actual state-of-art screening techniques of chronic myeloid leukemia, these results were, at microscale, at least 10 times faster than the reported detection methods for chronic myeloid leukemia. Concerning point-of-care applications, this work paves the way for other new and more complex versions of optical based genosensors.

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