Multi-site optical excitation using ChR2 and micro-LED array

N. Grossman, V. Poher, M.S. Grubb, G.T. Kennedy, K. Nikolic, B. McGovern, R. Berlinguer Palmini, E.M. Drakakis, M.A.A. Neil, M.D. Dawson, J. Burrone, P. Degenaar

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Studying neuronal processes such as synaptic summation, dendritic physiology and neural network dynamics requires complex spatiotemporal control over neuronal activities. The recent development of neural photosensitization tools, such as channelrhodopsin-2 (ChR2), offers new opportunities for non-invasive, flexible and cell-specific neuronal stimulation. Previously, complex spatiotemporal control of photosensitized neurons has been limited by the lack of appropriate optical devices which can provide 2D stimulation with sufficient irradiance. Here we present a simple and powerful solution that is based on an array of high-power micro light-emitting diodes (micro-LEDs) that can generate arbitrary optical excitation patterns on a neuronal sample with micrometre and millisecond resolution. We first describe the design and fabrication of the system and characterize its capabilities. We then demonstrate its capacity to elicit precise electrophysiological responses in cultured and slice neurons expressing ChR2.
LanguageEnglish
JournalJournal of Neural Engineering
Volume7
Issue number016004
DOIs
Publication statusPublished - 14 Jan 2009

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Photoexcitation
Neurons
Light emitting diodes
Photosensitivity Disorders
Light
Optical Devices
Physiology
Optical devices
Neural networks
Fabrication

Keywords

  • Multi-site optical excitation
  • ChR2
  • micro-LED array

Cite this

Grossman, N., Poher, V., Grubb, M. S., Kennedy, G. T., Nikolic, K., McGovern, B., ... Degenaar, P. (2009). Multi-site optical excitation using ChR2 and micro-LED array. Journal of Neural Engineering, 7(016004). https://doi.org/10.1088/1741-2560/7/1/016004
Grossman, N. ; Poher, V. ; Grubb, M.S. ; Kennedy, G.T. ; Nikolic, K. ; McGovern, B. ; Berlinguer Palmini, R. ; Drakakis, E.M. ; Neil, M.A.A. ; Dawson, M.D. ; Burrone, J. ; Degenaar, P. / Multi-site optical excitation using ChR2 and micro-LED array. In: Journal of Neural Engineering. 2009 ; Vol. 7, No. 016004.
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Grossman, N, Poher, V, Grubb, MS, Kennedy, GT, Nikolic, K, McGovern, B, Berlinguer Palmini, R, Drakakis, EM, Neil, MAA, Dawson, MD, Burrone, J & Degenaar, P 2009, 'Multi-site optical excitation using ChR2 and micro-LED array' Journal of Neural Engineering, vol. 7, no. 016004. https://doi.org/10.1088/1741-2560/7/1/016004

Multi-site optical excitation using ChR2 and micro-LED array. / Grossman, N.; Poher, V.; Grubb, M.S.; Kennedy, G.T.; Nikolic, K.; McGovern, B.; Berlinguer Palmini, R.; Drakakis, E.M.; Neil, M.A.A.; Dawson, M.D.; Burrone, J.; Degenaar, P.

In: Journal of Neural Engineering, Vol. 7, No. 016004, 14.01.2009.

Research output: Contribution to journalArticle

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AU - Poher, V.

AU - Grubb, M.S.

AU - Kennedy, G.T.

AU - Nikolic, K.

AU - McGovern, B.

AU - Berlinguer Palmini, R.

AU - Drakakis, E.M.

AU - Neil, M.A.A.

AU - Dawson, M.D.

AU - Burrone, J.

AU - Degenaar, P.

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Grossman N, Poher V, Grubb MS, Kennedy GT, Nikolic K, McGovern B et al. Multi-site optical excitation using ChR2 and micro-LED array. Journal of Neural Engineering. 2009 Jan 14;7(016004). https://doi.org/10.1088/1741-2560/7/1/016004