Mucus-on-a-chip: investigating the barrier properties of mucus with organic bioelectronics

Reece McCoy; Kaixin Wang; Jeremy Treiber; Ying Fu; George G. Malliaras; Alberto Salleo; Róisín M. Owens

doi:10.1039/d4tb01351d

Mucus-on-a-chip: investigating the barrier properties of mucus with organic bioelectronics

Reece McCoy, Kaixin Wang, Jeremy Treiber, Ying Fu, George G. Malliaras, Alberto Salleo, Róisín M. Owens^*

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Gastrointestinal (GI) mucus is a biologically complex hydrogel that acts as a partially permeable barrier between the contents of the GI tract and the mucosal epithelial lining. Its structural integrity is essential for the lubrication of the tract thereby aiding smooth transit of contents, and the protection of the epithelium from pathogens that seek to colonise and invade. Understanding its physical response to drugs and the microbiome is essential for treating many gastrointestinal infectious diseases. Given this, a static in vitro model of a GI mucus-on-a-chip has been developed with integrated electronics to monitor the barrier properties of mucus hydrogels. Its application for investigating the effect of drugs and biofilm formation on the mucus structure is validated using rheological techniques, confocal microscopy and electrochemical impedance spectroscopy (EIS).

Original language	English
Pages (from-to)	577-587
Number of pages	11
Journal	Journal of Materials Chemistry B
Volume	13
Issue number	2
Early online date	22 Nov 2024
DOIs	https://doi.org/10.1039/d4tb01351d
Publication status	E-pub ahead of print - 22 Nov 2024

Funding

R. M. was funded by an EPSRC Cambridge Nanoscience and Nanotechnology (NanoDTC) PhD studentship (Award EP/ S022953/1). Part of this work was performed at the Stanford Nano Shared Facilities (SNSF), supported by National Science Foundation under award ECCS-2026822. Part of this work was performed in part in the nano@Stanford laboratories, which are supported by the National Science Foundation as part of the National Nanotechnology Coordinated Infrastructure under award ECCS-2026822. This material is based upon work supported by the Air Force Office of Scientific Research under award no. FA8655-20-1-7021 to R. M. O. This work was supported by the Henry Royce Institute for advanced materials through the Equipment Access Scheme enabling access to the Wolfson Electron Microscopy Suite at Cambridge; Cambridge Royce facilities grant EP/P024947/1 and Sir Henry Royce Institute– recurrent grant (EP/R00661X/1). Y. F. acknowledges support from the Fellowship of Community for Analytical Measurement Science (CAMS, 600310/22/11).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

mucus
hydrogel
drugs and biofilm formation

Access to Document

10.1039/d4tb01351dLicence: CC BY 3.0

McCoy-etal-Mucus-on-a-chip-investigating-the-barrier-properties-of-mucus-with-organic-bioelectronicsFinal published version, 3.16 MBLicence: CC BY 3.0

Cite this

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abstract = "Gastrointestinal (GI) mucus is a biologically complex hydrogel that acts as a partially permeable barrier between the contents of the GI tract and the mucosal epithelial lining. Its structural integrity is essential for the lubrication of the tract thereby aiding smooth transit of contents, and the protection of the epithelium from pathogens that seek to colonise and invade. Understanding its physical response to drugs and the microbiome is essential for treating many gastrointestinal infectious diseases. Given this, a static in vitro model of a GI mucus-on-a-chip has been developed with integrated electronics to monitor the barrier properties of mucus hydrogels. Its application for investigating the effect of drugs and biofilm formation on the mucus structure is validated using rheological techniques, confocal microscopy and electrochemical impedance spectroscopy (EIS).",

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AU - Salleo, Alberto

AU - Owens, Róisín M.

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Mucus-on-a-chip: investigating the barrier properties of mucus with organic bioelectronics

Abstract

Funding

UN SDGs

Keywords

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