Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse

C.E. Lawrence, Y.Y.W. Paterson, S.H. Wright, P.A. Knight, H.R.P. Miller

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Abstract

The relationship between intestinal pathology and immune expulsion of gastrointestinal nematodes remains controversial. Immune expulsion of gastrointestinal helminth parasites is usually associated with Th2 responses, but the effector mechanisms directly responsible for parasite loss have not been elucidated. Mast cell hyperplasia is a hallmark of infection with gastrointestinal nematodes, in particular Trichinella spiralis. Although the precise mechanism by which mast cells induce expulsion of these parasites has not been elucidated, it has been proposed that mast cell mediators, including cytokines and granule chymases, act to create an environment inhospitable to the parasite, part of this being the induction of intestinal inflammation. Therefore, the aims of this study were to dissect the role of mast cells and mast cell proteases in the induction of parasite-induced enteropathy. Mast cell-deficient W/Wv and mouse mast cell protease-1 (mMCP-1)-deficient mice were infected with T. spiralis, and parasite expulsion, enteropathy, and Th2 responses were determined. Expulsion of the parasite was delayed in both strains of mice compared with wild-type controls; additionally, in both cases, the enteropathy was significantly ameliorated. Although Th2 responses were significantly reduced in mast cell-deficient W/Wv mice, those from mMCP-1-deficient mice were similar to wild-type mice. Additionally, levels of TNF-α and nitric oxide were significantly reduced in both W/Wv and mMCP-1 deficient mice. These results imply that mast cells may contribute to the induction of protective Th2 responses and, importantly, that the intestinal inflammation associated with gastrointestinal helminths is partly mediated by mMCP-1 MLN, mesenteric lymph node; MMC, mucosal mast cell; mMCP-1, mouse mast cell protease-1; MPO, myeloperoxidase; p.i., postinfection; TUNEL, terminal dUTP nick-end labeling; VCU, villus crypt unit
LanguageEnglish
Pages155-165
Number of pages10
JournalGastroenterology
Volume127
Issue number1
DOIs
Publication statusPublished - Jul 2004

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Helminths
Mast Cells
Parasites
Infection
Trichinella spiralis
Chymases
Inflammation
Mouse Mcpt1 protein
In Situ Nick-End Labeling
Peroxidase
Hyperplasia
Nitric Oxide
Peptide Hydrolases
Tumor Necrosis Factor-alpha
Lymph Nodes
Pathology
Cytokines

Keywords

  • gastroenterology

Cite this

Lawrence, C.E. ; Paterson, Y.Y.W. ; Wright, S.H. ; Knight, P.A. ; Miller, H.R.P. / Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse. In: Gastroenterology. 2004 ; Vol. 127, No. 1. pp. 155-165.
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Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse. / Lawrence, C.E.; Paterson, Y.Y.W.; Wright, S.H.; Knight, P.A.; Miller, H.R.P.

In: Gastroenterology, Vol. 127, No. 1, 07.2004, p. 155-165.

Research output: Contribution to journalArticle

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AU - Paterson, Y.Y.W.

AU - Wright, S.H.

AU - Knight, P.A.

AU - Miller, H.R.P.

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