Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

Jens Müller, Osman El-Maarri, Johannes Oldenburg, Bernd Pötzsch, G. Mayer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.
LanguageEnglish
Pages1033-1037
Number of pages4
JournalAnalytical and Bioanalytical Chemistry
Volume390
Issue number4
DOIs
Publication statusPublished - Feb 2008

Fingerprint

High performance liquid chromatography
Nucleic Acids
High Pressure Liquid Chromatography
Monitoring
Experiments
In Vitro Techniques

Keywords

  • aptamer
  • SELEX
  • dHPLC WAVE
  • spectroscopy
  • nucleic acids

Cite this

Müller, Jens ; El-Maarri, Osman ; Oldenburg, Johannes ; Pötzsch, Bernd ; Mayer, G. / Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. In: Analytical and Bioanalytical Chemistry. 2008 ; Vol. 390, No. 4. pp. 1033-1037.
@article{0f5d97d40b434d6fbbb10073681af459,
title = "Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography",
abstract = "Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.",
keywords = "aptamer, SELEX, dHPLC WAVE, spectroscopy, nucleic acids",
author = "Jens M{\"u}ller and Osman El-Maarri and Johannes Oldenburg and Bernd P{\"o}tzsch and G. Mayer",
year = "2008",
month = "2",
doi = "10.1007/s00216-007-1699-8",
language = "English",
volume = "390",
pages = "1033--1037",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
number = "4",

}

Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. / Müller, Jens; El-Maarri, Osman; Oldenburg, Johannes; Pötzsch, Bernd; Mayer, G.

In: Analytical and Bioanalytical Chemistry, Vol. 390, No. 4, 02.2008, p. 1033-1037.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

AU - Müller, Jens

AU - El-Maarri, Osman

AU - Oldenburg, Johannes

AU - Pötzsch, Bernd

AU - Mayer, G.

PY - 2008/2

Y1 - 2008/2

N2 - Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.

AB - Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.

KW - aptamer

KW - SELEX

KW - dHPLC WAVE

KW - spectroscopy

KW - nucleic acids

UR - http://dx.doi.org/10.1007/s00216-007-1699-8

U2 - 10.1007/s00216-007-1699-8

DO - 10.1007/s00216-007-1699-8

M3 - Article

VL - 390

SP - 1033

EP - 1037

JO - Analytical and Bioanalytical Chemistry

T2 - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 4

ER -