Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

Jens Müller, Osman El-Maarri, Johannes Oldenburg, Bernd Pötzsch, G. Mayer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences.
Original languageEnglish
Pages (from-to)1033-1037
Number of pages4
JournalAnalytical and Bioanalytical Chemistry
Volume390
Issue number4
DOIs
Publication statusPublished - Feb 2008

Keywords

  • aptamer
  • SELEX
  • dHPLC WAVE
  • spectroscopy
  • nucleic acids

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