Monitoring the assembly and aggregation of polypeptide materials by time-resolved emission spectra

Abeer Alghamdi, Li Hung C. Chung, Olaf J. Rolinski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Polypeptide assembly and aggregation are the common forms of its physiological and pathological activity, and monitoring them on a molecular level is critical for resolving numerous medical (e.g., onset of neurodegenerative diseases) or biological problems. Sensitivity of the intrinsic fluorescence of protein to its assembly, aggregation, or complexation offers a noninvasive methodology for identifying and determining different stages of these processes. In this protocol, we present the approach based on the time-resolved emission spectra (TRES), which reveals the number of fluorescent residues, the presence of dielectric relaxation, and the changes in fluorescence kinetics during aggregation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsMaxim G. Ryadnov
Place of PublicationNew York, NY
PublisherHumana Press
Pages167-177
Number of pages11
ISBN (Electronic)9781071609286
ISBN (Print)9781071609279
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2208
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • dielectric relaxation
  • fluorescence intensity decay
  • intrinsic fluorescence
  • protein assembly and aggregation
  • time-resolved emission spectra

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