Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples

P.A.G. Cormack, M.M. Ariffin, E.I. Miller, R.A. Anderson

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer. The recovery of a 50 ng diazepam standard spiked into blank hair was 93%, with good precision (RSD = 1.5%). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively. The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself. The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC−MS−MS method.
LanguageEnglish
Pages256-262
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number1
DOIs
Publication statusPublished - 2007

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Diazepam
Metabolites
Polymers
Benzodiazepines
Blood
Recovery
Pharmaceutical Preparations

Keywords

  • solid-phase extraction
  • diazepam
  • metabolites

Cite this

Cormack, P.A.G. ; Ariffin, M.M. ; Miller, E.I. ; Anderson, R.A. / Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples. In: Analytical Chemistry. 2007 ; Vol. 79, No. 1. pp. 256-262.
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abstract = "An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer. The recovery of a 50 ng diazepam standard spiked into blank hair was 93{\%}, with good precision (RSD = 1.5{\%}). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively. The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself. The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC−MS−MS method.",
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Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples. / Cormack, P.A.G.; Ariffin, M.M.; Miller, E.I.; Anderson, R.A.

In: Analytical Chemistry, Vol. 79, No. 1, 2007, p. 256-262.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecularly imprinted solid-phase extraction of diazepam and its metabolites from hair samples

AU - Cormack, P.A.G.

AU - Ariffin, M.M.

AU - Miller, E.I.

AU - Anderson, R.A.

PY - 2007

Y1 - 2007

N2 - An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer. The recovery of a 50 ng diazepam standard spiked into blank hair was 93%, with good precision (RSD = 1.5%). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively. The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself. The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC−MS−MS method.

AB - An anti-diazepam, molecularly imprinted polymer (MIP) has been synthesized and used to extract diazepam and other benzodiazepines from hair samples via a molecularly imprinted solid-phase extraction (MISPE) protocol. Optimum retention of diazepam on the MIP columns was achieved using an apolar solvent, and the binding capacity of the polymer toward diazepam was found to be 110 ng of diazepam/mg of polymer. The recovery of a 50 ng diazepam standard spiked into blank hair was 93%, with good precision (RSD = 1.5%). The LOD and LOQ of diazepam in spiked hair samples were 0.09 and 0.14 ng/mg, respectively. The MISPE method was demonstrated to be applicable to the analysis of diazepam metabolites and other benzodiazepine drugs, in addition to diazepam itself. The application of the extraction method to postmortem hair samples yielded results that were in good agreement with the corresponding ELISA data (from blood samples) and data arising from the analysis of the same blood samples using a validated in-house SPE-LC−MS−MS method.

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KW - diazepam

KW - metabolites

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JO - Analytical Chemistry

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