Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices

A. Beltran, N. Fontanals, R.M. Marce, P.A.G. Cormack, F. Borrull

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the present paper, we describe the synthesis of a cephalexin (CFX) molecularly imprinted polymer (MIP), the direct application of the MIP to SPE for the determination of CFX (which is a -lactam antibiotic) in human urine and the use of the MIP in a tandem SPE system to determine CFX in river water. The molecularly imprinted polymers (MIP) showed cross-selectivity for amoxicillin (AMX; also a -lactam antibiotic). This allowed both CFX and AMX to be quantified in acidified human urine, with recoveries of 78 and 60% for CFX and AMX, respectively, when the urine samples were spiked with CFX and AMX at 4 mg/L. These analyses were facile because the molecularly imprinted solid-phase extraction (MISPE) extracts were clear compared to the nonpurified samples. In order to increase the sample volume for river water analyses, a tandem SPE system incorporating a commercially available sorbent was implemented. With this set-up, CFX was determined with recoveries in excess of 50% when 200 mL of acidified river water samples spiked at 10 g/L with CFX were percolated through the tandem system.
LanguageEnglish
Pages3319-3326
Number of pages7
JournalJournal of Separation Science
Volume32
Issue number19
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Cephalexin
Polymers
Lactams
Water
Rivers
Antibiotics
Anti-Bacterial Agents
Recovery
Amoxicillin
Sorbents

Keywords

  • amoxicillin
  • cephalexin
  • molecularly imprinted polymer
  • river water samples
  • urine samples

Cite this

Beltran, A. ; Fontanals, N. ; Marce, R.M. ; Cormack, P.A.G. ; Borrull, F. / Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. In: Journal of Separation Science. 2009 ; Vol. 32, No. 19. pp. 3319-3326.
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Molecularly imprinted solid-phase extraction of cephalexin from water-based matrices. / Beltran, A.; Fontanals, N.; Marce, R.M.; Cormack, P.A.G.; Borrull, F.

In: Journal of Separation Science, Vol. 32, No. 19, 10.2009, p. 3319-3326.

Research output: Contribution to journalArticle

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AU - Fontanals, N.

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