Molecularly imprinted monodisperse microspheres for competitive radioassay

Lei Ye, Peter A. G. Cormack, Klaus Mosbach

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the ‘classical’ particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.

LanguageEnglish
Pages35-38
Number of pages4
JournalAnalytical Communications
Volume36
DOIs
Publication statusPublished - 1999

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Theophylline
Hydrophobicity
Sorbents
Microspheres
Estradiol
Polymers
Binding Sites
Polymerization

Cite this

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abstract = "In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the ‘classical’ particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.",
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Molecularly imprinted monodisperse microspheres for competitive radioassay. / Ye, Lei; Cormack, Peter A. G.; Mosbach, Klaus.

In: Analytical Communications , Vol. 36, 1999, p. 35-38.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecularly imprinted monodisperse microspheres for competitive radioassay

AU - Ye, Lei

AU - Cormack, Peter A. G.

AU - Mosbach, Klaus

PY - 1999

Y1 - 1999

N2 - In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the ‘classical’ particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.

AB - In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the ‘classical’ particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.

U2 - 10.1039/A809014I

DO - 10.1039/A809014I

M3 - Article

VL - 36

SP - 35

EP - 38

JO - Analytical Communications

T2 - Analytical Communications

JF - Analytical Communications

SN - 1359-7337

ER -