Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose

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Abstract

A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
Original languageEnglish
Pages (from-to)2245-2254
Number of pages10
JournalSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume57
Issue number11
DOIs
Publication statusPublished - 14 Sep 2001

Keywords

  • lifetime sensing
  • FRET
  • glucose
  • affinity sensor
  • diabetes mellitus
  • nanoscience

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