Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose

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15 Citations (Scopus)

Abstract

A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Förster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
LanguageEnglish
Pages2245-2254
Number of pages10
JournalSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume57
Issue number11
DOIs
Publication statusPublished - 14 Sep 2001

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resonance fluorescence
Concanavalin A
glucose
Glucose
affinity
Assays
energy transfer
dextrans
Dextran
Dextrans
Distribution functions
Fluorescence
distribution functions
fluorescence
sensitivity
sensors
Sensors
decay
Fluorescence Resonance Energy Transfer
malachite green

Keywords

  • lifetime sensing
  • FRET
  • glucose
  • affinity sensor
  • diabetes mellitus
  • nanoscience

Cite this

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abstract = "A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional F{\"o}rster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.",
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AU - Rolinski, O.J.

AU - Birch, D.J.S.

AU - McCartney, L.

AU - Pickup, J.C.

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KW - FRET

KW - glucose

KW - affinity sensor

KW - diabetes mellitus

KW - nanoscience

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JO - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

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JF - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

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