Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture

S.S. Soares; L. Pedro; G.N.M. Ferreira

doi:10.1109/ENBENG.2012.6331354

Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture

S.S. Soares^*, L. Pedro, G.N.M. Ferreira

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

Abstract

Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.

Original language	English
Title of host publication	2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)
Place of Publication	Piscataway, NJ
Publisher	IEEE
Number of pages	6
ISBN (Electronic)	9781467345262, 9781467345255
ISBN (Print)	9781467345248
DOIs	https://doi.org/10.1109/ENBENG.2012.6331354
Publication status	Published - 18 Oct 2012
Externally published	Yes
Event	2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012 - Coimbra, Portugal Duration: 23 Feb 2012 → 25 Feb 2012

Conference

Conference	2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012
Country/Territory	Portugal
City	Coimbra
Period	23/02/12 → 25/02/12

Funding

The authors thank the financial support from Fundacao para a Ciencia e Tecnologia (FCT), through contract PTDC/BI0/69682/2006, and to CBME/IBB, LA, FEDER/POCI 2010. S.S. Soares and L. Pedro also thank the financial support from FCT, through grants SFRH/BPD/30290/2006 and SFRH/BD/36674/2007, respectively.

Keywords

in vitro assembly
molecular therapy
purification
tropism manipulation
virus-like particles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/ENBENG.2012.6331354

Cite this

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title = "Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture",

abstract = "Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.",

keywords = "in vitro assembly, molecular therapy, purification, tropism manipulation, virus-like particles",

author = "S.S. Soares and L. Pedro and G.N.M. Ferreira",

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doi = "10.1109/ENBENG.2012.6331354",

language = "English",

isbn = "9781467345248",

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T1 - Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture

AU - Soares, S.S.

AU - Pedro, L.

AU - Ferreira, G.N.M.

PY - 2012/10/18

Y1 - 2012/10/18

N2 - Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.

AB - Protein nanoparticles, such as virus-like particles (VLPs), consist only of the virus shell without any viral genetic information packaged inside. Similarly to viruses, the structural proteins that comprise a VLP can spontaneously self-assemble to form the particle or can assemble through several intermediate steps. This work addresses the characterization, manipulation and purification of a chimeric simian-human immunodeficiency VLP constructed by fusion of SIV matrix protein (p17) and HIV-1 p6 accessory protein. This fusion protein assembles as spherical nanoparticles of about 80 nm in diameter that are released to the culture media when expressed in HEK 293T cells. A simple two-step purification process was used to purify these nanoparticles. Also, different approaches - multiple-transfections or chemical coupling - were performed to target manipulation. Finally, a new approach for the production of these virus-like particles is described where the structural protein subunits are used and their assembly is promoted in vitro.

KW - in vitro assembly

KW - molecular therapy

KW - purification

KW - tropism manipulation

KW - virus-like particles

U2 - 10.1109/ENBENG.2012.6331354

DO - 10.1109/ENBENG.2012.6331354

M3 - Conference contribution book

AN - SCOPUS:84869455226

SN - 9781467345248

BT - 2012 IEEE 2nd Portuguese Meeting in Bioengineering (ENBENG)

PB - IEEE

CY - Piscataway, NJ

T2 - 2012 IEEE 2nd Portuguese Meeting in Bioengineering, ENBENG 2012

Y2 - 23 February 2012 through 25 February 2012

ER -

Molecular determinants of virus-like nanoparticle assembly in vitro and in animal cell culture

Abstract

Conference

Funding

Keywords

UN SDGs

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Cite this